Proteomic analysis of the carotenogenic yeast Xanthophyllomyces dendrorhous.
Proteomic analysis of the carotenogenic yeast Xanthophyllomyces dendrorhous.
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DOI：
10.1186/1471-2180-11-131
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发表时间：
2011-06-13
期刊：
影响因子：
4.2
通讯作者：
Cifuentes V
中科院分区：
文献类型：
--
作者：
Martinez-Moya P;Watt SA;Niehaus K;Alcaíno J;Baeza M;Cifuentes V
该酵母用于微生物生产抗氧化剂类胡萝卜素虾青素。在这项研究中，我们建立了一种最佳的蛋白质提取方法，并首次对菌株ATCC 24230进行了蛋白质组学分析。用双向凝胶电泳法测定诱导胡萝卜素形成前和诱导过程中的蛋白质组分，并用质谱仪鉴定蛋白质组分。在观察到的大约600个蛋白质点中，鉴定出131个非冗余蛋白质。蛋白质组学分析使我们能够识别出50个差异表达的蛋白质，这些蛋白质属于几个具有不同表达模式的类别。这些分析表明，在胡萝卜素合成之前，与乙酰-辅酶A合成相关的酶更丰富。后来，氧化还原和应激相关的蛋白在胡萝卜素的诱导过程中上调。对于类胡萝卜素生物合成酶甲氧丙酸激酶和植烯/角鲨烯合成酶，我们观察到在类胡萝卜素的诱导和/或积累过程中有更高的丰度。此外，经典的抗氧化物酶，如过氧化氢酶、谷胱甘肽过氧化物酶和胞质超氧化物歧化酶都没有被鉴定出来。我们的结果提供了潜在重要的胡萝卜素发生相关蛋白质的概述，其中包括与碳水化合物和脂肪生物合成途径有关的蛋白质，以及几个与氧化还原和胁迫相关的蛋白质。此外，这些结果可能表明，在有氧条件下，树枝杆菌会积累虾青素，以清除代谢过程中产生的活性氧(ROS)。
The yeast Xanthophyllomyces dendrorhous is used for the microbiological production of the antioxidant carotenoid astaxanthin. In this study, we established an optimal protocol for protein extraction and performed the first proteomic analysis of the strain ATCC 24230. Protein profiles before and during the induction of carotenogenesis were determined by two-dimensional polyacrylamide gel electrophoresis and proteins were identified by mass spectrometry. Among the approximately 600 observed protein spots, 131 non-redundant proteins were identified. Proteomic analyses allowed us to identify 50 differentially expressed proteins that fall into several classes with distinct expression patterns. These analyses demonstrated that enzymes related to acetyl-CoA synthesis were more abundant prior to carotenogenesis. Later, redox- and stress-related proteins were up-regulated during the induction of carotenogenesis. For the carotenoid biosynthetic enzymes mevalonate kinase and phytoene/squalene synthase, we observed higher abundance during induction and/or accumulation of carotenoids. In addition, classical antioxidant enzymes, such as catalase, glutathione peroxidase and the cytosolic superoxide dismutases, were not identified. Our results provide an overview of potentially important carotenogenesis-related proteins, among which are proteins involved in carbohydrate and lipid biosynthetic pathways as well as several redox- and stress-related proteins. In addition, these results might indicate that X. dendrorhous accumulates astaxanthin under aerobic conditions to scavenge the reactive oxygen species (ROS) generated during metabolism.
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影响因子：
4.4
作者：
KOBAYASHI, M;KAKIZONO, T;NAGAI, S
通讯作者：
NAGAI, S
影响因子：
3.5
作者：
Hümbelin, M;Thomas, A;Berry, A
通讯作者：
Berry, A
影响因子：
2.9
作者：
Hayduk, EJ;Choe, LH;Lee, KH
通讯作者：
Lee, KH
影响因子：
3.2
作者：
FUGE, EK;BRAUN, EL;WERNERWASHBURNE, M
通讯作者：
WERNERWASHBURNE, M
影响因子：
7.4
作者：
Grünewald, K;Eckert, M;Hagen, C
通讯作者：
Hagen, C