A Custom Ultra-Low-Cost 3D Bioprinter Supports Cell Growth and Differentiation.
A Custom Ultra-Low-Cost 3D Bioprinter Supports Cell Growth and Differentiation.
复制标题
定制的超低成本三维生物打印机支持细胞生长和分化。
DOI：
10.3389/fbioe.2020.580889
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发表时间：
2020
影响因子：
5.7
通讯作者：
Taraviras S
中科院分区：
文献类型：
--
作者：
Ioannidis K;Danalatos RI;Champeris Tsaniras S;Kaplani K;Lokka G;Kanellou A;Papachristou DJ;Bokias G;Lygerou Z;Taraviras S
3D生物打印的进步已经允许将干细胞沿着生物材料和生长因子用于新的组织工程方法。然而，这些系统连同其消耗品的成本沿着目前非常高，限制了它们的适用性。为了解决这个问题，我们将3D打印机转换为开源3D生物打印机，并基于可访问的藻酸盐/明胶前体生产定制的生物墨水，从而实现了具有成本效益的解决方案。分析了生物打印机的分辨率，包括线宽、铺展比和挤出均匀性测量，沿着生物墨水的流变特性，揭示了在可印刷性窗口内的高生物打印精度。在生物打印过程之后，在HeLa京都和HEK 293 T细胞系上验证细胞存活和增殖。此外，我们分离并3D生物打印了来自小鼠脑室下区的出生后神经干细胞祖细胞以及来自小鼠骨髓的间充质干细胞。我们的研究结果表明，我们的低成本3D生物打印机可以支持两种不同类型的原代干细胞群体的细胞增殖和分化，这表明它可以作为开发干细胞研究和组织工程的有效研究模型的可靠工具。
Advances in 3D bioprinting have allowed the use of stem cells along with biomaterials and growth factors toward novel tissue engineering approaches. However, the cost of these systems along with their consumables is currently extremely high, limiting their applicability. To address this, we converted a 3D printer into an open source 3D bioprinter and produced a customized bioink based on accessible alginate/gelatin precursors, leading to a cost-effective solution. The bioprinter’s resolution, including line width, spreading ratio and extrusion uniformity measurements, along with the rheological properties of the bioinks were analyzed, revealing high bioprinting accuracy within the printability window. Following the bioprinting process, cell survival and proliferation were validated on HeLa Kyoto and HEK293T cell lines. In addition, we isolated and 3D bioprinted postnatal neural stem cell progenitors derived from the mouse subventricular zone as well as mesenchymal stem cells derived from mouse bone marrow. Our results suggest that our low-cost 3D bioprinter can support cell proliferation and differentiation of two different types of primary stem cell populations, indicating that it can be used as a reliable tool for developing efficient research models for stem cell research and tissue engineering.
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影响因子：
9
作者：
Gao T;Gillispie GJ;Copus JS;Pr AK;Seol YJ;Atala A;Yoo JJ;Lee SJ
通讯作者：
Lee SJ
影响因子：
14
作者：
Crompton, K. E.;Goud, J. D.;Forsythe, J. S.
通讯作者：
Forsythe, J. S.
影响因子：
6.6
作者：
Chung, Johnson H. Y.;Naficy, Sina;Wallace, Gordon G.
通讯作者：
Wallace, Gordon G.
影响因子：
2.9
作者：
Li, Minggan;Tian, Xiaoyu;Chen, Xiongbiao
通讯作者：
Chen, Xiongbiao
DOI：
10.1007/s11693-013-9116-4
发表时间：
2013-09-01
期刊：
Systems and synthetic biology
影响因子：
--
作者：
Landrain, Thomas;Meyer, Morgan;Sussan, Remi
通讯作者：
Sussan, Remi