Purification and activity of the first recombinant enzyme for biodegrading hepatotoxin by Sphingopyxis sp. USTB-05
Purification and activity of the first recombinant enzyme for biodegrading hepatotoxin by Sphingopyxis sp. USTB-05
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首个鞘氨醇生物降解肝毒素重组酶的纯化和活性。
DOI：
10.1016/j.algal.2020.101863
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发表时间：
2020-05
影响因子：
5.1
通讯作者：
Yan Hai
中科院分区：
文献类型：
--
作者：
Xu Qianqian;Ma Hongfei;Zhang Haiyang;Fan Jinhui;Yin Chunhua;Liu Xiaolu;Liu Yang;Wang Huasheng;Yan Hai
Cyanobacterial hepatotoxins which are produced and released by bloom-forming cyanobacteria may threaten the safety of drinking water for human beings and animals. Microcystinase (MlrA) catalyzes the first and most important step for biodegrading hepatotoxic microcystins (MCs) and nodularin (NOD), which hydrolyzes the cyclic hepatotoxin into the linear hepatotoxin as the first product. Here the recombinant MlrA ofSphingopyxissp. USTB-05 for biodegrading hepatotoxins was firstly purified and its activity was also investigated. The purification process of MlrA was comprised of four steps, affinity purification of glutathione S-transferase (GST)-tagged MlrA, thrombin cleavage of GST tag, GST tag removal and thrombin removal with the benzamidine sepharose. The purified recombinant MlrA was found to have a strong ability to catalyze hepatotoxins, and initial microcystin-LR (MC-LR) of 19.9 mg/L and NOD of 25.2 mg/L were removed within 10 min and 12 h at the protein concentration of 60 mg/L, respectively. The results indicated that the enzyme MlrA with a high activity could be purified and obtained with the biotechnology process of expression and purification described here, which is very important in further studies on the enzyme structure and the enzymatic mechanism for biodegrading hepatotoxins.
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影响因子：
8.8
作者：
Yan, Hai;Wang, Junfeng;Wang, Hui
通讯作者：
Wang, Hui
影响因子：
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作者：
Pearson L;Mihali T;Moffitt M;Kellmann R;Neilan B
通讯作者：
Neilan B
影响因子：
8.8
作者：
Tsuji, Kiyomi;Asakawa, Miki;Harada, Ken-ichi
通讯作者：
Harada, Ken-ichi
影响因子：
4.1
作者：
Dziga, Dariusz;Wasylewski, Marcin;Wladyka, Benedykt
通讯作者：
Wladyka, Benedykt
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影响因子：
3
作者：
Chunhua Yin;Hui Wang;QIanqian Xu;Le Lv
通讯作者：
Le Lv