Human Mig chemokine: biochemical and functional characterization.
Human Mig chemokine: biochemical and functional characterization.
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DOI：
10.1084/jem.182.5.1301
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发表时间：
1995-11-01
影响因子：
15.3
通讯作者：
Farber, Joshua M.
中科院分区：
文献类型：
--
作者：
Liao, Fang;Rabin, Ronald L.;Yannelli, John R.;Koniaris, Leonidas G.;Vanguri, Padmavathy;Farber, Joshua M.
Mig 是 CXC 亚家族的趋化因子，是通过对由淋巴因子激活的巨噬细胞制备的 cDNA 文库进行差异筛选而发现的。 mig 基因可在巨噬细胞和其他细胞中诱导，以响应干扰素 (IFN)-γ。我们用编码人 Mig 的 cDNA 转染了中国仓鼠卵巢 (CHO) 细胞，并衍生了 CHO 细胞系，从中纯化了重组人 Mig (rHuMig)。 rHuMig 诱导人肿瘤浸润 T 淋巴细胞 (TIL) 和培养的活化人外周血来源淋巴细胞中 [Ca2+]i 短暂升高。在人中性粒细胞、单核细胞或 Epstein-Barr 病毒转化的 B 淋巴母细胞系中未观察到任何反应。通过改良的博伊登室测定，rHuMig 对 TIL 具有趋化性，但 rHuMig 对中性粒细胞或单核细胞没有趋化性。 SDS-PAGE显示，CHO细胞系、IFN-γ处理的人外周血单核细胞和IFN-γ处理的人单核细胞系THP-1的细胞均分泌多种且相同的HuMig种类。使用 CHO 衍生的 rHuMig，我们发现该物种的异质性是由于碱性羧基末端残基处的蛋白水解切割造成的，并且蛋白水解发生在 CHO 细胞分泌 rHuMig 之前而不是之后。分泌型 rHuMig 的主要种类长度范围为 78 至 103 个氨基酸，后者对应于从 HuMig cDNA 预测的全长分泌蛋白。在钙流测定中，与全长rHuMig相比，rHuMig的羧基末端截短形式具有较低的比活性，并且截短的种类不会阻断全长种类的活性。 HuMig 很可能在 T 细胞运输中发挥作用，也可能在活化 T 细胞生理学的其他方面发挥作用。
Mig is a chemokine of the CXC subfamily that was discovered by differential screening of a cDNA library prepared from lymphokine- activated macrophages. The mig gene is inducible in macrophages and in other cells in response to interferon (IFN)-gamma. We have transfected Chinese hamster ovary (CHO) cells with cDNA encoding human Mig and we have derived CHO cell lines from which we have purified recombinant human Mig (rHuMig). rHuMig induced the transient elevation of [Ca2+]i in human tumor-infiltrating T lymphocytes (TIL) and in cultured, activated human peripheral blood-derived lymphocytes. No responses were seen in human neutrophils, monocytes, or Epstein-Barr virus-transformed B lymphoblastoid cell lines. rHuMig was chemotactic for TIL by a modified Boyden chamber assay but rHuMig was not chemotactic for neutrophils or monocytes. The CHO cell lines, IFN-gamma-treated human peripheral-blood monocytes, and IFN-gamma-treated cells of the human monocytic cell line THP-1 all secreted multiple and identical HuMig species as revealed by SDS-PAGE. Using the CHO-derived rHuMig, we have shown that the species' heterogeneity is due to proteolytic cleavage at basic carboxy-terminal residues, and that the proteolysis occurs before and not after rHuMig secretion by the CHO cells. The major species of secreted rHuMig ranged from 78 to 103 amino acids in length, the latter corresponding to the full-length secreted protein predicted from the HuMig cDNA. Carboxy-terminal-truncated forms of rHuMig were of lower specific activity compared to full-length rHuMig in the calcium flux assay, and the truncated species did not block the activity of the full- length species. It is likely that HuMig plays a role in T cell trafficking and perhaps in other aspects of the physiology of activated T cells.
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影响因子：
2.9
作者：
BLUM, H;BEIER, H;GROSS, HJ
通讯作者：
GROSS, HJ
影响因子：
3.7
作者：
DENISON, MR;ZOLTICK, PW;WEISS, SR
通讯作者：
WEISS, SR
DOI：
10.1006/bbrc.1993.1403
发表时间：
1993-04-15
影响因子：
3.1
作者：
FARBER, JM
通讯作者：
FARBER, JM
DOI：
10.1073/pnas.87.14.5238
发表时间：
1990-07-01
影响因子：
11.1
作者：
FARBER, JM
通讯作者：
FARBER, JM
影响因子：
2.2
作者：
FALK, W;GOODWIN, RH;LEONARD, EJ
通讯作者：
LEONARD, EJ