GMR biosensor arrays: a system perspective.
GMR biosensor arrays: a system perspective.
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DOI：
10.1016/j.bios.2010.01.038
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发表时间：
2010-05-15
影响因子：
12.6
通讯作者：
Wang SX
中科院分区：
文献类型：
--
作者：
Hall DA;Gaster RS;Lin T;Osterfeld SJ;Han S;Murmann B;Wang SX
巨磁阻式生物传感器在灵敏的、可量化的生物分子检测中正变得越来越普遍。然而，为了使磁性生物传感与当前的光学蛋白质微阵列技术竞争，需要增加传感器的数量，同时保持较小阵列(1-8个传感器)的高灵敏度和快速读出时间特性。在本文中，我们提出了一种可扩展的电路结构，可用于更大的传感器阵列(可单独寻址的传感器)，同时保持高读出速率(在不到4秒内扫描整个阵列)。为了达到该扫描速率，该系统利用了时间域多路复用和频域多路复用两者。在实现上，我们提出了一种新的电路结构，它不使用经典的惠斯通电桥来测量传感器的电阻微小变化。取而代之的是，采用了围绕跨阻抗放大器设计的架构。对该体系结构进行了详细的分析，包括噪声、失真和潜在的误差源，然后提出了整个系统的全局优化策略，该系统包括磁性标签、传感器和接口电子设备。为了证明灵敏度，对两个浓度未知的盲加样本进行了定量检测，检测浓度低于酶联免疫吸附试验的检测限值。最后，通过实时监测由不同浓度的三种独特蛋白质功能化的传感器，证明了该系统的多解性和重复性。
Giant magnetoresistive biosensors are becoming more prevalent for sensitive, quantifiable biomolecular detection. However, in order for magnetic biosensing to become competitive with current optical protein microarray technology, there is a need to increase the number of sensors while maintaining the high sensitivity and fast readout time characteristic of smaller arrays (1 – 8 sensors). In this paper, we present a circuit architecture scalable for larger sensor arrays (64 individually addressable sensors) while maintaining a high readout rate (scanning the entire array in less than 4 seconds). The system utilizes both time domain multiplexing and frequency domain multiplexing in order to achieve this scan rate. For the implementation, we propose a new circuit architecture that does not use a classical Wheatstone bridge to measure the small change in resistance of the sensor. Instead, an architecture designed around a transimpedance amplifier is employed. A detailed analysis of this architecture including the noise, distortion, and potential sources of errors is presented, followed by a global optimization strategy for the entire system comprising the magnetic tags, sensors, and interface electronics. To demonstrate the sensitivity, quantifiable detection of two blindly spiked samples of unknown concentrations has been performed at concentrations below the limit of detection for the enzyme-linked immunosorbent assay. Lastly, the multipexability and reproducibility of the system was demonstrated by simultaneously monitoring sensors functionalized with three unique proteins at different concentrations in real-time.
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影响因子：
12.6
作者：
de Boer, B. M.;Kahlman, J. A. H. M.;Veen, J.
通讯作者：
Veen, J.
影响因子：
12.6
作者：
Baselt, DR;Lee, GU;Colton, RJ
通讯作者：
Colton, RJ
影响因子：
7.4
作者：
Millen, Rachel L.;Nordling, John;Granger, Michael C.
通讯作者：
Granger, Michael C.
影响因子：
12.6
作者：
Xu, Liang;Yu, Heng;Wang, Shan X.
通讯作者：
Wang, Shan X.
DOI：
10.1073/pnas.0810822105
发表时间：
2008-12-30
影响因子：
11.1
作者：
Osterfeld, Sebastian J.;Yu, Heng;Wang, Shan X.
通讯作者：
Wang, Shan X.