In vitro characterization of xeno-free clinically relevant human collagen and its applicability in cell-laden 3D bioprinting.
In vitro characterization of xeno-free clinically relevant human collagen and its applicability in cell-laden 3D bioprinting.
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临床相关的无异种人胶原蛋白的体外表征及其在细胞3D生物打印中的适用性。
DOI：
10.1177/0885328220959162
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发表时间：
2021-03
影响因子：
2.9
通讯作者：
Kishore, Vipuil
中科院分区：
文献类型：
--
作者：
Schmitt, Trevor;Kajave, Nilabh;Cai, Huan Huan;Gu, Linxia;Albanna, Mohammad;Kishore, Vipuil
I型胶原蛋白通常来源于异种来源，被广泛用作组织工程应用的生物材料。然而，异种胶原的使用通常与已知影响细胞反应和重塑的机械、结构和生物学特性的物种特异性变化相关。此外，免疫并发症和疾病传播的风险也是主要关切。本研究的目的是表征一种新的无异种人皮肤来源的胶原蛋白，并评估其作为生物墨水用于细胞负载3D生物打印的适用性。四种不同浓度的人胶原蛋白（即，0.5 1 mg/mL、lmg/mL、3 mg/mL和6 mg/mL）用于胶原水凝胶的合成。此外，牛胶原蛋白被用作异种对照。SDS-PAGE分析结果显示存在α1、α2和β链，证实分离后I型人胶原蛋白的完整性得以保持。聚合速率和压缩模量随着人胶原蛋白浓度的增加而显著增加。当比较两种不同来源的胶原蛋白时，异种胶原蛋白的聚合速率显著快于人胶原蛋白（p < 0.05），而压缩模量相当。拉曼光谱显示在1600 cm −1附近的酰胺I带中有一个大峰，表明人胶原蛋白水凝胶中存在致密和超组织的纤维状结构。相反，异种胶原蛋白的酰胺I条带强度与酰胺II和酰胺III条带的强度相当。此外，使用6 mg/mL人胶原蛋白作为生物墨水产生了具有高形状保真度和细胞活力的3D打印构建体。另一方面，异种胶原蛋白未能产生稳定的3D打印结构。总之，这项研究的结果为使用人源胶原蛋白作为异种来源的可行替代品提供了动力，用于组织工程应用的临床相关支架的3D生物打印。
Collagen type I, commonly derived from xenogenic sources, is extensively used as a biomaterial for tissue engineering applications. However, the use of xenogenic collagen is typically associated with species specific variation in mechanical, structural, and biological properties that are known to influence cellular response and remodeling. In addition, immunological complications and risks of disease transmission are also major concerns. The goal of this study is to characterize a new xeno-free human skin-derived collagen and assess its applicability as a bioink for cell-laden 3D bioprinting. Four different concentrations of human collagen (i.e., 0.5 mg/mL, 1 mg/mL, 3 mg/mL and 6 mg/mL) were employed for the synthesis of collagen hydrogels. In addition, bovine collagen was used as a xenogenic control. Results from SDS-PAGE analysis showed the presence of α1, α2, and β chains, confirming that the integrity of type I human collagen is maintained post isolation. Polymerization rate and compressive modulus increased significantly with increase in the concentration of human collagen. When comparing two different sources of collagen, the polymerization rate of xenogenic collagen was significantly faster (p < 0.05) than human collagen while the compressive modulus was comparable. Raman spectroscopy showed a large peak in the Amide I band around 1600cm−1, indicating a dense and supraorganized fibrillar structure in human collagen hydrogels. Conversely, Amide I band intensity for xenogenic collagen was comparable to that of Amide II and Amide III bands. Further, the use of 6 mg/mL human collagen as a bioink yielded 3D printed constructs with high shape fidelity and cell viability. On the other hand, xenogenic collagen failed to yield stable 3D printed constructs. Together, the results from this study provides an impetus for using human-derived collagen as a viable alternative to xenogenic sources for 3D bioprinting of clinically relevant scaffolds for tissue engineering applications.
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影响因子：
13.6
作者：
Hinton TJ;Jallerat Q;Palchesko RN;Park JH;Grodzicki MS;Shue HJ;Ramadan MH;Hudson AR;Feinberg AW
通讯作者：
Feinberg AW
影响因子：
4.6
作者：
Filardo, G.;Petretta, M.;Grigolo, B.
通讯作者：
Grigolo, B.
影响因子：
5
作者：
Achilli, Matteo;Mantovani, Diego
通讯作者：
Mantovani, Diego
影响因子：
4.1
作者：
Browne, Shane;Zeugolis, Dimitrios I.;Pandit, Abhay
通讯作者：
Pandit, Abhay
影响因子：
3.8
作者：
Chimene, David;Lennox, Kimberly K.;Gaharwar, Akhilesh K.
通讯作者：
Gaharwar, Akhilesh K.