Shear stress and material surface effects on adherent human monocyte apoptosis.
Shear stress and material surface effects on adherent human monocyte apoptosis.
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剪切应力和材料表面对贴壁人单核细胞凋亡的影响。
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
James M. Anderson
中科院分区：
文献类型：
--
作者：
M. Shive;W. Brodbeck;E. Colton;James M. Anderson
单核细胞作为吞噬细胞和炎症反应介质在预防心血管器械相关感染中发挥关键作用。然而，这些器械的持续感染仍会发生，并且可能归因于单核细胞在动态流动环境中与异物相互作用导致的有害细胞改变。因此，研究了剪切应力和粘附到材料表面对人单核细胞凋亡的影响。旋转圆盘系统产生生理相关的剪切应力水平（0-14 dyn/cm（2）），剪切相关的细胞凋亡发生在粘附单核细胞的特点。使用膜联蛋白V分析，在剪切下4小时，随着剪切以接近线性的方式增加，多聚腺苷酸粘附的单核细胞的凋亡增加至>70%的水平（r2 = 0.713）。使用共聚焦显微镜定性证实，丝状（F）-肌动蛋白分布改变，DNA片段化发生，并激活半胱天冬酶参与剪切诱导的细胞凋亡。静态研究确定，自发性细胞凋亡是材料依赖性超过72小时，证明与烷基改性的玻璃相比，粘附到聚氨酯的单核细胞凋亡之间的显着差异。随着时间的推移，TNF-α治疗以剂量依赖性方式增强了这种材料依赖性。TNF-α处理的细胞的F-肌动蛋白含量降低至未处理细胞的<62%。我们得出结论，材料表面和动态流动的伴随效应介导人单核细胞凋亡，并可能在植入的心血管器械感染的背景下产生严重影响。
Monocytes play a critical role as both phagocytes and mediators of inflammatory responses in the prevention of cardiovascular device-related infections. However, persistent infection of these devices still occurs and may be attributed to deleterious cellular alterations resulting from monocyte interactions with a foreign material in an environment of dynamic flow. Thus, the effects of both shear stress and adhesion to material surfaces on human monocyte apoptosis were investigated. A rotating disk system generated physiologically relevant shear stress levels (0-14 dyn/cm(2)), and shear-related apoptosis occurring in adherent monocytes was characterized. Using annexin V analysis, apoptosis of polyurethane-adherent monocytes under shear for 4 h increased to levels >70% with increasing shear in a near-linear fashion (r2 = 0.713). It was qualitatively confirmed using confocal microscopy that filamentous (F)-actin distribution was altered, that DNA fragmentation occurred, and that activated caspases were involved in shear-induced apoptosis. Static studies determined that spontaneous apoptosis was material-dependent over 72 h by demonstrating marked differences between apoptosis of monocytes adherent to a polyurethane compared to an alkyl-modified glass. Treatment with TNF-alpha augmented this material dependency in a dose-dependent fashion over time. F-actin content of TNF-alpha-treated cells decreased to <62% of untreated cells. We conclude that concomitant effects from both material surfaces and dynamic flow mediate human monocyte apoptosis and may have serious implications in the context of implanted cardiovascular device infection.
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DOI：
10.1161/01.atv.15.1.2
发表时间：
1995
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
P. Walpola;A. Gotlieb;M. Cybulsky;B. Langille
通讯作者：
P. Walpola;A. Gotlieb;M. Cybulsky;B. Langille
影响因子：
3.6
作者：
ANDERSON, JM;ZIATS, NP;BONFIELD, TL
通讯作者：
BONFIELD, TL
血小板介导的表皮葡萄球菌与疏水性 NHLBI 参考聚乙烯的粘附。
DOI：
10.1002/jbm.820270902
发表时间：
1993
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
Wang,IW;Anderson,JM;Marchant,RE
通讯作者：
Marchant,RE
脂多糖、肿瘤坏死因子-α 和 IL-1β 可防止人外周血单核细胞中的程序性细胞死亡（细胞凋亡）。
DOI：
--
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Mangan,DF;Welch,GR;Wahl,SM
通讯作者：
Wahl,SM
影响因子：
7.5
作者：
Shyy, JYJ;Chien, S
通讯作者：
Chien, S