Histological Lesions and Replication Sites of PCV3 in Naturally Infected Pigs.
Histological Lesions and Replication Sites of PCV3 in Naturally Infected Pigs.
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DOI：
10.3390/ani11061520
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发表时间：
2021-05-24
期刊：
影响因子：
--
通讯作者：
Vannucci FA
中科院分区：
文献类型：
--
作者：
De Conti ER;Resende TP;Marshall-Lund L;Rovira A;Vannucci FA
诊断猪圆环病毒 3 型 (PCV3) 是养猪业的一项挑战。尽管该病毒最近已被分离出来，但 PCV3 相关的组织学病变模式仍有待阐明。本研究描述了通过原位杂交在组织学病变内的 PCV3 mRNA 与通过实时 PCR 在自然感染的猪中检测到的 PCV3 DNA 之间的关联。与PCV3 mRNA检测相关的主要组织学病变分别是心脏和肺的淋巴浆细胞性心肌炎和淋巴浆细胞性间质性肺炎。我们的研究结果为与 PCV3 相关的微观病变提供了强有力的指导，这可能在 PCV3 诊断中发挥关键作用。猪圆环病毒 3 型 (PCV3) 最近被描述为猪流产和全身性血管炎的潜在原因。尽管通过实时 PCR 在世界各国的几种猪组织中检测到了该病毒，但与 PCV3 相关的疾病尚未得到令人满意的澄清。本研究的目的是调查在自然感染的猪中通过原位杂交 (ISH) 检测到的组织学病变内 PCV3 mRNA 的存在与通过实时 PCR 检测到的 PCV3 DNA 之间的关联。总共分析了 25 例 PCV3 PCR 阳性病例。对这些病例的福尔马林固定组织进行组织学损伤和 PCV3 ISH-RNA 阳性信号的评估。最常见的组织病理学病变组织类型是心脏，占 76.2%，其中最常见的病变为淋巴浆细胞性心肌炎和心外膜炎。淋巴浆细胞性间质性肺炎也很常见，占 47.6%。肾脏、肝脏、脾脏和淋巴结也有病变。 PCV3-ISH-RNA 阳性信号主要与包括动脉在内的各种组织中的淋巴浆细胞炎症浸润相关。根据我们的结果，提交用于组织病理学和 mRNA 原位杂交以确认或排除 PCV3 诊断的最小样本集是心脏、肺和淋巴组织（即脾脏和淋巴结），特别是用于与 PCV2 相关疾病相关的鉴别诊断。
Diagnosing porcine circovirus type 3 (PCV3) is a challenge in pig production. Although the virus has been recently isolated, the patterns of PCV3-associated histological lesions are still to be elucidated. The present study describes the association of PCV3 mRNA by in situ hybridization within histological lesions and PCV3 DNA detected by real-time PCR in naturally infected pigs. The main histologic lesions associated with PCV3 mRNA detection were lymphoplasmacytic myocarditis and lymphoplasmacytic interstitial pneumonia, in heart and lung, respectively. Our findings offer robust guidance of microscopic lesions associated with PCV3, which may have a key role in PCV3 diagnosis. Porcine circovirus type 3 (PCV3) has been recently described as a potential cause of abortions and systemic vasculitis in pigs. Although the virus has been detected by real-time PCR in several porcine tissues from countries worldwide, PCV3-associated diseases have not been satisfactorily clarified. The objective of this study was to investigate the association between the presence of PCV3 mRNA detected by in situ hybridization (ISH) within histological lesions and PCV3 DNA detected by real-time PCR in naturally infected pigs. A total of 25 PCV3 PCR-positive cases were analyzed. Formalin-fixed tissues from these cases were evaluated for histologic lesions and for ISH-RNA positive signals for PCV3. The most frequent tissue type with histopathologic lesions was heart, 76.2%, with lymphoplasmacytic myocarditis and epicarditis as the most frequent lesions observed. Lymphoplasmacytic interstitial pneumonia was also a frequent finding, 47.6%. There were also lesions in kidney, liver, spleen and lymph nodes. PCV3-ISH-RNA positive signals were mostly observed in association with lymphoplasmacytic inflammatory infiltrate in various tissues, including arteries. Based on our results, the minimum set of specimens to be submitted for histopathology and mRNA in situ hybridization to confirm or exclude a diagnosis of PCV3 are heart, lung and lymphoid tissues (i.e., spleen and lymph nodes), especially for differential diagnosis related with PCV2-associated diseases.
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