Enhanced reactive oxygen detoxification occurs in salt-stressed soybean roots expressing GmSALT3.
Enhanced reactive oxygen detoxification occurs in salt-stressed soybean roots expressing GmSALT3.
复制标题
表达GmSALT3的盐胁迫大豆根中活性氧解毒作用增强。
DOI：
10.1111/ppl.13709
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发表时间：
2022-05
影响因子：
6.4
通讯作者：
Gilliham, Matthew
中科院分区：
文献类型：
--
作者：
Qu, Yue;Guan, Rongxia;Yu, Lili;Berkowitz, Oliver;David, Rakesh;Whelan, James;Ford, Melanie;Wege, Stefanie;Qiu, Lijuan;Gilliham, Matthew
大豆（Glycine max）是世界上重要的粮食和食用油作物。大豆植物对盐（NaCl）敏感，在盐条件下报道产量显著降低。GmSALT 3基因是大豆耐盐性的显性基因。GmSALT 3编码属于植物阳离子/质子交换器（CHX）家族的跨膜蛋白，并且在盐和非盐条件下主要在根韧皮部和木质部相关细胞中表达。目前尚不清楚ER定位的GmSALT 3通过何种分子机制有助于耐盐性，因为其定位排除了直接参与离子排斥。为了深入了解潜在的分子机制，我们使用了来自两个大豆NIL（近等基因系）的根的RNA测序分析; NIL-S（盐敏感性，Gmsalt 3）和NIL-T（耐盐性，GmSALT 3），在三个时间点（0小时、6小时和3天）在对照和盐条件（200 mM NaCl）下生长。基因本体论（GO）分析表明，NIL-T具有更大的响应与氧化还原。在NIL-T中，ROS含量较低，清除酶活性较高，与RNA-seq数据一致。进一步的分析表明，盐处理后，与钙信号传导、囊泡运输和凯氏带（CS）发育相关的基因在NIL-T中上调。我们认为，GmSALT 3通过防止ROS在根中过度积累，并可能调节Ca 2+信号传导、囊泡运输和扩散屏障的形成，提高了NIL‐T科普盐胁迫的能力。
Soybean (Glycine max) is an important crop globally for food and edible oil production. Soybean plants are sensitive to salinity (NaCl), with significant yield decreases reported under saline conditions. GmSALT3 is the dominant gene underlying a major QTL for salt tolerance in soybean. GmSALT3 encodes a transmembrane protein belonging to the plant cation/proton exchanger (CHX) family, and is predominately expressed in root phloem and xylem associated cells under both saline and non‐saline conditions. It is currently unknown through which molecular mechanism(s) the ER‐localised GmSALT3 contributes to salinity tolerance, as its localisation excludes direct involvement in ion exclusion. In order to gain insights into potential molecular mechanism(s), we used RNA‐seq analysis of roots from two soybean NILs (near isogenic lines); NIL‐S (salt‐sensitive, Gmsalt3), and NIL‐T (salt‐tolerant, GmSALT3), grown under control and saline conditions (200 mM NaCl) at three time points (0 h, 6 h, and 3 days). Gene ontology (GO) analysis showed that NIL‐T has greater responses aligned to oxidation reduction. ROS were less abundant and scavenging enzyme activity was greater in NIL‐T, consistent with the RNA‐seq data. Further analysis indicated that genes related to calcium signalling, vesicle trafficking and Casparian strip (CS) development were upregulated in NIL‐T following salt treatment. We propose that GmSALT3 improves the ability of NIL‐T to cope with saline stress through preventing ROS overaccumulation in roots, and potentially modulating Ca2+ signalling, vesicle trafficking and formation of diffusion barriers.
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