Luminescent gold nanoparticles with pH-dependent membrane adsorption.
Luminescent gold nanoparticles with pH-dependent membrane adsorption.
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DOI：
10.1021/ja201930p
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发表时间：
2011-07-27
影响因子：
15
通讯作者：
Zheng, Jie
中科院分区：
文献类型：
--
作者：
Yu, Mengxiao;Zhou, Chen;Liu, Jinbin;Hankins, Julia D.;Zheng, Jie
pH值调节许多细胞过程，也是疾病进展的指标。因此，pH响应材料通常作为细胞生物学基础理解的工具或疾病诊断和治疗的药物。虽然金纳米颗粒在生物医学上有广泛的应用，但由于非特异性血清蛋白吸附，它们很少在天然生物环境中与活细胞表现出pH依赖的相互作用。在此，我们报告了通过在发光金纳米粒子上涂覆天然肽谷胱甘肽和最简单的稳定的氨基硫醇半胱胺，我们使纳米粒子不仅具有高的抗血清蛋白吸附能力，而且在血清蛋白存在的情况下也能在活细胞膜上表现出pH依赖性吸附。将这种依赖pH的膜吸附行为结合到金纳米颗粒中，可能会催化金属纳米颗粒在生物过程的基础理解以及涉及pH变化的疾病诊断和治疗方面的新的生物医学应用。
pH regulates many cellular processes and is also an indicator of disease progression. Therefore, pH responsive materials often serve as either tools in the fundamental understanding of cell biology or medicines for disease diagnosis and therapy. While gold nanoparticles have found broad biomedical applications, very few of them exhibit pH dependent interactions with live cells in a native biological environment due to nonspecific serum protein adsorption. Herein, we report that by coating luminescent gold nanoparticles with a natural peptide, glutathione and the simplest stable aminothiol, cysteamine, we enabled the nanoparticles to exhibit not only high resistance to serum protein adsorption but also pH dependent adsorption onto the live cell membrane in the presence of serum proteins. Incorporating this pH dependent membrane adsorption behavior into gold nanoparticles could potentially catalyze new biomedical applications of metal nanoparticles in the fundamental understanding of biological processes as well as disease diagnosis and therapy, where pH changes are involved.
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影响因子：
15
作者：
Richards CI;Hsiang JC;Senapati D;Patel S;Yu J;Vosch T;Dickson RM
通讯作者：
Dickson RM
影响因子：
13.3
作者：
Kong, Tao;Zeng, Jie;Xing, James Z.
通讯作者：
Xing, James Z.
DOI：
10.1073/pnas.0710575105
发表时间：
2008-04-15
影响因子：
11.1
作者：
Keren, S.;Zavaleta, C.;Gambhir, S. S.
通讯作者：
Gambhir, S. S.
DOI：
10.1073/pnas.0601463103
发表时间：
2006-04-25
影响因子：
11.1
作者：
Reshetnyak, YK;Andreev, OA;Engelman, DM
通讯作者：
Engelman, DM
影响因子：
4.9
作者：
Gullotti E;Yeo Y
通讯作者：
Yeo Y