PDGF, TGF-beta, and heterotypic cell-cell interactions mediate endothelial cell-induced recruitment of 10T1/2 cells and their differentiation to a smooth muscle fate.
PDGF, TGF-beta, and heterotypic cell-cell interactions mediate endothelial cell-induced recruitment of 10T1/2 cells and their differentiation to a smooth muscle fate.
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DOI：
10.1083/jcb.141.3.805
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发表时间：
1998-05-04
期刊：
影响因子：
--
通讯作者：
D'Amore PA
中科院分区：
文献类型：
--
作者：
Hirschi KK;Rohovsky SA;D'Amore PA
我们的目标是确定内皮细胞(EC)是否以及如何在血管形成过程中招募平滑肌细胞和周细胞的前体并诱导其分化。多能胚胎10T1/2细胞被认为是壁细胞前体。在琼脂糖共培养中，EC通过血小板衍生生长因子BB诱导10T1/2细胞迁移。与EC共培养的10T1/2细胞由多角形变为梭形，使人联想到培养中的平滑肌细胞。免疫组织化学和Western印迹分析检测10T1/2细胞在无EC和有EC存在的情况下，平滑肌(SM)特异性标记物的表达。在单独培养的10T1/2细胞中未检测到SM-肌球蛋白、SM-22α和降钙素蛋白的表达，而在与EC共同培养的10T1/2细胞中，这三种SM特异性蛋白的表达均被显著诱导。用转化生长因子-β处理10T1/2细胞后，细胞的表型和SM标志物的变化与共培养时相似。在共培养中中和转化生长因子-β可阻断SM标志物的表达和细胞形态的改变。为了评估10T1/2细胞在体内对发育中的血管壁的贡献能力，将预先标记的10T1/2细胞种植在胶原基质中，并将其植入小鼠皮下。荧光标记的细胞结合到发育中的血管的内层，在那里它们表达SM标记。这些体外和体内的观察揭示了在血管发育过程中发生的细胞-细胞相互作用，以及在其中概述发育过程的病理学。
We aimed to determine if and how endothelial cells (EC) recruit precursors of smooth muscle cells and pericytes and induce their differentiation during vessel formation. Multipotent embryonic 10T1/2 cells were used as presumptive mural cell precursors. In an under-agarose coculture, EC induced migration of 10T1/2 cells via platelet-derived growth factor BB. 10T1/2 cells in coculture with EC changed from polygonal to spindle-shaped, reminiscent of smooth muscle cells in culture. Immunohistochemical and Western blot analyses were used to examine the expression of smooth muscle (SM)-specific markers in 10T1/2 cells cultured in the absence and presence of EC. SM-myosin, SM22α, and calponin proteins were undetectable in 10T1/2 cells cultured alone; however, expression of all three SM-specific proteins was significantly induced in 10T1/2 cells cocultured with EC. Treatment of 10T1/2 cells with TGF-β induced phenotypic changes and changes in SM markers similar to those seen in the cocultures. Neutralization of TGF-β in the cocultures blocked expression of the SM markers and the shape change. To assess the ability of 10T1/2 cells to contribute to the developing vessel wall in vivo, prelabeled 10T1/2 cells were grown in a collagen matrix and implanted subcutaneously into mice. The fluorescently marked cells became incorporated into the medial layer of developing vessels where they expressed SM markers. These in vitro and in vivo observations shed light on the cell–cell interactions that occur during vessel development, as well as in pathologies in which developmental processes are recapitulated.
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DOI：
10.1083/jcb.89.2.379
发表时间：
1981-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chamley-Campbell JH;Campbell GR;Ross R
通讯作者：
Ross R
DOI：
10.1016/0045-6039(82)90014-8
发表时间：
1982-01-01
期刊：
CELL DIFFERENTIATION
影响因子：
--
作者：
EKBLOM, P;SARIOLA, H;SAXEN, L
通讯作者：
SAXEN, L
影响因子：
2.9
作者：
DUBAND, JL;GIMONA, M;SMALL, JV
通讯作者：
SMALL, JV
影响因子：
3.7
作者：
BORRIONE, AC;ZANELLATO, AMC;SARTORE, S
通讯作者：
SARTORE, S
影响因子：
7.8
作者：
BLANK, RS;THOMPSON, MM;OWENS, GK
通讯作者：
OWENS, GK