Analysis of epitope recognition of antibodies induced by DNA immunization against hemagglutinin protein of influenza A virus.
Analysis of epitope recognition of antibodies induced by DNA immunization against hemagglutinin protein of influenza A virus.
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甲型流感病毒血凝素蛋白DNA免疫诱导抗体表位识别分析。
DOI：
10.1016/s0264-410x(03)00257-3
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发表时间：
2003
期刊：
影响因子：
5.5
通讯作者：
M. Itoh
中科院分区：
文献类型：
--
作者：
K. Tonegawa;E. Nobusawa;K. Nakajima;Takashi Kato;T. Kutsuna;K. Kuroda;Toshikatsu Shibata;Y. Harada;A. Nakamura;M. Itoh
为了寻找有效的DNA候选疫苗，利用基因枪将甲型流感病毒血凝素（HA）基因及其衍生突变体的cDNA注射到小鼠体内。用HA1 DNA（含或不含膜结构域）免疫的小鼠均表现出体液免疫应答，抵抗同源病毒攻击的存活率与注射HA DNA的小鼠相当。为了分析基因枪免疫诱导的抗体识别的表位，我们采用了嵌合HA蛋白的结合试验方法。用HA DNA免疫小鼠的血清抗体能识别HA1结构域，但不能识别HA2结构域。此外，用HA1 DNA免疫小鼠获得的抗血清与HA1结构域上的每个已知抗原位点发生反应，与HA DNA免疫获得的结果相似。
In an effort to find efficient DNA vaccine candidates, cDNA of influenza A virus hemagglutinin (HA) gene and several derived mutants were injected into mice using a gene gun. Mice immunized with HA1 DNA, with or without a membrane domain, showed a humoral immune response and the survival rate against homologous virus challenge was comparable to that of mice injected with HA DNA. In order to analyze epitopes recognized by antibodies induced by gene gun immunization, we used a binding assay employing the chimeric HA protein method. Serum antibodies of mice immunized with HA DNA recognized the HA1 domain but not the HA2 domain. In addition, antisera obtained from mice immunized with HA1 DNA reacted with each of the known antigenic sites on the HA1 domain, similar to the results obtained with HA DNA immunization.
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DNA 免疫：DNA 表达的血凝素的分泌对抗体反应的影响。
影响因子：
5.5
作者：
Torres,CA;Yang,K;Mustafa,F;Robinson,HL
通讯作者：
Robinson,HL
DOI：
10.1073/pnas.90.24.11478
发表时间：
1993-12-15
影响因子：
11.1
作者：
FYNAN, EF;WEBSTER, RG;ROBINSON, HL
通讯作者：
ROBINSON, HL
编码流感血凝素的DNA作为禽流感疫苗的用途。
影响因子：
3.1
作者：
Fynan,EF;Robinson,HL;Webster,RG
通讯作者：
Webster,RG
影响因子：
5.5
作者：
WEBSTER, RG;FYNAN, EF;ROBINSON, H
通讯作者：
ROBINSON, H