The neuropeptide Y receptor gene repository, phylogeny and comparative expression in allotetraploid common carp.
The neuropeptide Y receptor gene repository, phylogeny and comparative expression in allotetraploid common carp.
复制标题
异源四倍体鲤鱼神经肽Y受体基因库、系统发育及比较表达
DOI：
10.1038/s41598-022-13587-2
复制
发表时间：
2022-06-08
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
NPY家族受体属于G蛋白偶联受体（GPCR），为内源性食欲刺激因子神经肽Y及其相关肽的跨膜转运奠定了生理基础。在本研究中，我们对10个代表性物种的npyr基因进行了研究，从异源四倍体C. carpio，C.鲤鱼的亚基因组数目是其B类二倍体黄竹枝孔无须鳕的两倍。系统发育分析表明，所有npyr基因被分为3个亚群，根据选择压力分析，它们都经历了强烈的纯化选择。随后的同线性分析表明，大多数npyr基因均匀分布在异源四倍体C.鲤鱼，其中NPY1R和NPY2R分别为串联重复。此外，npyr基因在异源四倍体C. Carpio提示npyr基因在免疫和生殖中的潜在功能。在成体组织中，npyr基因主要分布在脑、性腺和皮肤中，其表达模式与C.鲤B亚基因组和黄竹坚鲤。本研究为进一步研究异源四倍体C.鲤鱼和脊椎动物。
NPY-family receptors belong to G protein-coupled receptors (GPCR), which lays a physiological foundation for the transmembrane transport of an endogenous appetite-stimulating factor neuropeptide Y and related peptides. In this study, we investigated the npyr genes in ten representative species, and twelve npyr genes were identified from allotetraploid C. carpio, the npyr gene number of C. carpio was twice the number of its subgenome B progenitor-like diploid Poropuntius huangchuchieni. Phylogenetic analysis showed that all npyr genes were divided into three subgroups, and they underwent strong purifying selection according to selection pressure analysis. Subsequently, synteny analysis showed that most npyr genes were evenly distributed on the homologous chromosomes of two subgenomes in allotetraploid C. carpio, in which npy1r and npy2r were tandem duplicated, respectively. In addition, the global expression of npyr genes during embryonic development in allotetraploid C. carpio suggested the potential function of npyr genes in immunity and reproduction. In adult tissues, npyr genes were mainly distributed in the brain, gonad, and skin, which displayed a similar expression pattern between the C. carpio B subgenome and P. huangchuchieni. In general, our research could provide reference information for future exploration of the NPY receptors and neuroendocrine system of allotetraploid C. carpio and vertebrates.
登录
查看更多内容
影响因子：
2.9
作者：
Gonzalez-Stegmaier, Roxana;Villarroel-Espindola, Franz;Romero, Alex
通讯作者：
Romero, Alex
影响因子：
3.4
作者：
Larsson TA;Olsson F;Sundstrom G;Lundin LG;Brenner S;Venkatesh B;Larhammar D
通讯作者：
Larhammar D
影响因子：
3
作者：
de Pedro, N;López-Patiño, MA;Alonso-Bedate, M
通讯作者：
Alonso-Bedate, M
影响因子：
3.9
作者：
Fredriksson, R;Larson, ET;Larhammar, D
通讯作者：
Larhammar, D
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro