In vivo molecular evaluation of guinea pig skin incisions healing after surgical suture and laser tissue welding using Raman spectroscopy.
In vivo molecular evaluation of guinea pig skin incisions healing after surgical suture and laser tissue welding using Raman spectroscopy.
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DOI：
10.1016/j.jphotobiol.2009.06.004
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发表时间：
2009-09-04
影响因子：
5.4
通讯作者：
Alfano, R. R.
中科院分区：
文献类型：
--
作者：
Alimova, A.;Chakraverty, R.;Muthukattil, R.;Elder, S.;Katz, A.;Sriramoju, V.;Lipper, Stanley;Alfano, R. R.
关键词：
通过使用拉曼光谱，在分子水平上评估豚鼠皮肤手术切口后的愈合过程。通过缝合或激光组织焊接（LTW）关闭切口后，分别确定拉曼光谱的差异。研究确定，酰胺III （1247 cm−1）带的拉曼峰与1326 cm−1（弹性蛋白和角蛋白带的叠加）的峰值之比可以用来评估伤口愈合的进展。在LTW和缝合皮肤中观察到酰胺I波段（1633 cm−1 ~ 1682 cm−1）的构象变化和1450 cm−1 ~ 1520 cm−1的光谱变化。以组织病理学为金标准，采用拉曼光谱法评价豚鼠皮肤LTW和缝合后愈合过程的各个阶段。LTW皮肤比缝合皮肤愈合得更好，表现出最小的角化过度，最小的胶原沉积，接近正常的表面轮廓，最小的真皮附着物损失。采用小波分解-重建基线校正算法去除拉曼光谱中的荧光翼。
The healing process in guinea pig skin following surgical incisions was evaluated at the molecular level, in vivo, by the use of Raman spectroscopy. After the incisions were closed either by suturing or by laser tissue welding (LTW), differences in the respective Raman spectra were identified. The study determined that the ratio of the Raman peaks of the amide III (1247 cm−1) band to a peak at 1326 cm−1 (the superposition of elastin and keratin bands) can be used to evaluate the progression of wound healing. Conformational changes in the amide I band (1633 cm−1 to 1682 cm−1) and spectrum changes in the range of 1450 cm−1 to 1520 cm−1 were observed in LTW and sutured skin. The stages of the healing process of the guinea pig skin following LTW and suturing were evaluated by Raman spectroscopy, using histopathology as the gold standard. LTW skin demonstrated better healing than sutured skin, exhibiting minimal hyperkeratosis, minimal collagen deposition, near-normal surface contour, and minimal loss of dermal appendages. A wavelet decomposition-reconstruction baseline correction algorithm was employed to remove the fluorescence wing from the Raman spectra.
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影响因子：
9
作者：
LEVENSON, SM;GEEVER, EF;ROSEN, H
通讯作者：
ROSEN, H
DOI：
10.1016/0031-6865(94)00050-6
发表时间：
1995-04-01
期刊：
Pharmaceutica acta Helvetiae
影响因子：
--
作者：
Ferry, L L;Argentieri, G;Lochner, D H
通讯作者：
Lochner, D H
DOI：
10.1073/pnas.0402933101
发表时间：
2004-06-22
影响因子：
11.1
作者：
Gnanakaran, S;Hochstrasser, RM;García, AE
通讯作者：
García, AE
影响因子：
--
作者：
Greve, Tanja M.;Andersen, Kristine B.;Nielsen, Ole F.
通讯作者：
Nielsen, Ole F.
DOI：
10.1089/clm.2000.18.3
发表时间：
2000-02-01
期刊：
JOURNAL OF CLINICAL LASER MEDICINE & SURGERY
影响因子：
--
作者：
Tang, J;Zeng, FN;Alfano, RR
通讯作者：
Alfano, RR