Investigating the healing mechanisms of an angiogenesis-promoting topical treatment for diabetic wounds using multimodal microscopy.
Investigating the healing mechanisms of an angiogenesis-promoting topical treatment for diabetic wounds using multimodal microscopy.
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DOI：
10.1002/jbio.201700195
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发表时间：
2018-03
影响因子：
2.8
通讯作者：
Boppart SA
中科院分区：
文献类型：
--
作者：
Li J;Bower AJ;Arp Z;Olson EJ;Holland C;Chaney EJ;Marjanovic M;Pande P;Alex A;Boppart SA
皮肤伤口愈合受损是糖尿病的一种重要并存状态，由微循环不良等因素引起。研究表明，血管生成是糖尿病伤口愈合过程中的关键步骤，在低氧条件下可以促进血管生成。在这项研究中，一种促进血管生成的糖尿病创面局部治疗，通过抑制脯氨酸羟基酶导致低氧诱导因子(HIF)的升高或维持，通过模拟低氧环境来促进血管生成，利用配备了相位变化光学相干断层扫描(PV-OCT)和荧光寿命成像显微镜(FLIM)的定制多模显微镜系统进行了研究。用PV-OCT跟踪微血管网络的再生，用FOLIM评估愈合过程中小鼠表皮角质形成细胞对治疗的体内代谢反应。结果显示，细胞内还原型烟酰胺腺嘌呤二核苷酸(NADH)的荧光寿命显著缩短，表明创面皮肤处于类缺氧环境，随后用PV-OCT评估血管密度的定量增加。在这些研究中获得的见解可能会为评估伤口愈合药物的有效性和愈合机制带来新的终点，因为在愈合延迟的情况下，无法使用现有的评估方法。
Impaired skin wound healing is a significant co-morbid condition of diabetes that is caused by poor microcirculation, among other factors. Studies have shown that angiogenesis, a critical step in the wound healing process in diabetic wounds, can be promoted under hypoxia. In this study, an angiogenesis-promoting topical treatment for diabetic wounds, which promotes angiogenesis by mimicking a hypoxic environment via inhibition of prolyl hydroxylase resulting in elevation or maintenance of hypoxia inducible factor (HIF), was investigated utilizing a custom-built multimodal microscopy system equipped with phase-variance optical coherence tomography (PV-OCT) and fluorescence lifetime imaging microscopy (FLIM). PV-OCT was used to track the regeneration of the microvasculature network, and FLIM was used to assess the in vivo metabolic response of mouse epidermal keratinocytes to the treatment during healing. Results show a significant decrease in the fluorescence lifetime of intracellular reduced nicotinamide adenine dinucleotide (NADH), suggesting a hypoxic-like environment in the wounded skin, followed by a quantitative increase in blood vessel density assessed by PV-OCT. Insights gained in these studies could lead to new endpoints for evaluation of the efficacy and healing mechanisms of wound-healing drugs in a setting where delayed healing does not permit available methods for evaluation to take place.
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影响因子：
3.8
作者：
An L;Qin J;Wang RK
通讯作者：
Wang RK
DOI：
10.1073/pnas.0708425104
发表时间：
2007-12-04
影响因子：
11.1
作者：
Skala, Melissa C.;Riching, Kristin M.;Ramanujam, Nirmala
通讯作者：
Ramanujam, Nirmala
无标记的体内细胞级检测和凋亡的成像。
影响因子：
2.8
作者：
Bower, Andrew J.;Marjanovic, Marina;Zhao, Youbo;Li, Joanne;Chaney, Eric J.;Boppart, Stephen A.
通讯作者：
Boppart, Stephen A.
影响因子：
3.4
作者：
Apelian, Clement;Harms, Fabrice;Boccara, A. Claude
通讯作者：
Boccara, A. Claude
影响因子：
3.5
作者：
Farhat, Golnaz;Mariampillai, Adrian;Kolios, Michael C.
通讯作者：
Kolios, Michael C.