Laminin Functionalized Biomimetic Nanofibers For Nerve Tissue Engineering.
Laminin Functionalized Biomimetic Nanofibers For Nerve Tissue Engineering.
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DOI：
10.1166/jbt.2013.1110
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发表时间：
2013-08-01
影响因子：
0.1
通讯作者：
Yu X
中科院分区：
文献类型：
--
作者：
Junka R;Valmikinathan CM;Kalyon DM;Yu X
由于缺乏合适的移植物、细胞渗透和修复不足，大间隙周围神经损伤对神经再生提出了重大挑战。仿生纳米纤维支架，功能化的表面与细胞外基质蛋白，可以导致新的疗法修复和再生受损的周围神经。在这里，从聚（己内酯）（PCL）和壳聚糖的共混物静电纺丝纳米纤维支架制造。利用壳聚糖上的胺基，通过碳二亚胺交联将层粘连蛋白功能化。交联允许层粘连蛋白以相对高的浓度附着在PCL-壳聚糖纳米纤维的表面，这是使用常规吸附方法不可能实现的。测试纳米纤维网的润湿性、机械性能以及细胞附着和增殖。壳聚糖与PCL的共混物提供了更有利的表面附着的雪旺细胞由于相比，纯PCL的接触角的减少。在具有交联层粘连蛋白的PCL-壳聚糖支架上生长的雪旺细胞的增殖率显著高于具有吸附层粘连蛋白的PCL-壳聚糖纳米纤维基质的增殖率。PCL-壳聚糖支架通过层粘连蛋白的交联具有改性表面，可以潜在地用作具有优异的机械和表面性质的多功能基底，用于神经组织工程应用的体内细胞递送。
Large-gap peripheral nerve injuries present a significant challenge for nerve regeneration due to lack of suitable grafts, insufficient cell penetration, and repair. Biomimetic nanofibrous scaffolds, functionalized on the surface with extracellular matrix proteins, can lead to novel therapies for repair and regeneration of damaged peripheral nerves. Here, nanofibrous scaffolds electrospun from blends of poly(caprolactone) (PCL) and chitosan were fabricated. Taking advantage of the amine groups on the chitosan, the surface of the scaffolds were functionalized with laminin by carbodiimide based crosslinking. Crosslinking allowed laminin to be attached to the surfaces of the PCL-chitosan nanofibers at relatively high concentrations that were not possible using conventional adsorption methods. The nanofibrous meshes were tested for wettability, mechanical properties and cell attachment and proliferation. Blending of chitosan with PCL provided more favorable surfaces for attachment of Schwann cells due to the reduction of the contact angle in comparison to neat PCL. Proliferation rates of Schwann cells grown on PCL-chitosan scaffolds with crosslinked laminin were significantly higher than the rates for PCL-chitosan nanofibrous matrices with adsorbed laminin. PCL-chitosan scaffolds with modified surfaces via crosslinking of laminin could potentially serves as versatile substrates with excellent mechanical and surface properties for in vivo cell delivery for nerve tissue engineering applications.
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