Relationships among gingival crevicular fluid biomarkers, clinical parameters of periodontal disease, and the subgingival microbiota.
Relationships among gingival crevicular fluid biomarkers, clinical parameters of periodontal disease, and the subgingival microbiota.
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DOI：
10.1902/jop.2009.090397
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发表时间：
2010-01
影响因子：
4.3
通讯作者：
Haffajee A
中科院分区：
文献类型：
--
作者：
Teles R;Sakellari D;Teles F;Konstantinidis A;Kent R;Socransky S;Haffajee A
目的是测量牙周健康和牙周炎受试者的龈沟液（GCF）生物标志物和龈下细菌种类的水平，以探讨这些生物标志物，龈下微生物群和牙周疾病的临床参数之间的关系。对20例牙周炎患者和20例牙周健康者的每颗牙的6个牙周临床参数进行了测量。GCF和龈下菌斑样本从每颗牙齿的近颊面获得。采用棋盘免疫印迹法测定GCF中白细胞介素-1 β（IL-1β）、基质金属蛋白酶-8（MMP-8）和IL-8的水平，并采用棋盘DNA-DNA杂交法定量40种细菌分类群的水平。分别分析每组的“临床健康”（CH）部位子集。使用非配对t检验或Mann-Whitney检验确定组间差异的显著性。使用斯皮尔曼等级相关系数确定免疫学、微生物学和临床数据之间的相关性。3种生物标志物的平均水平和平均临床参数与橙子和红色复合物种的比例呈正相关（p<0.05）。牙周炎患者的CH部位IL-1β和IL-8水平高于牙周健康者的CH部位，橙子和红色复合物的比例也高于牙周健康者的CH部位（p<0.05）。红色复合物与所有生物标志物的表达呈正相关（p<0.05），而紫色和黄色复合物与IL-1β和IL-8呈负相关（p<0.05）。牙周炎受试者的CH站点比牙周健康受试者的CH站点呈现更高水平的GCF生物标志物和牙周病原体。不同的微生物复合体表现出与特定GCF生物标志物的不同关联。
The objectives were to measure the levels of gingival crevicular fluid (GCF) biomarkers and subgingival bacterial species in periodontally healthy and periodontitis subjects in order to explore relations among these biomarkers, the subgingival microbiota, and clinical parameters of periodontal disease. Clinical periodontal parameters were measured at 6 sites per tooth in 20 periodontitis and 20 periodontally healthy subjects. GCF and subgingival plaque samples were obtained from the mesiobuccal aspect of every tooth. GCF levels of interleukin-1β (IL-1β), matrix metalloproteinase-8 (MMP-8) and IL-8 were measured using checkerboard immunoblotting and the levels of 40 bacterial taxa quantified using checkerboard DNA-DNA hybridization. A subset of “clinically healthy” (CH) sites from each group was analyzed separately. Significance of differences between groups was determined using the unpaired t-test or the Mann-Whitney test. Correlations among immunological, microbiological and clinical data were determined using the Spearman rank correlation coefficient. There were positive correlations among mean clinical parameters and mean levels of the 3 biomarkers and proportions of Orange and Red complex species (p<0.05). CH sites from periodontitis subjects had higher levels of IL-1β and IL-8 and higher proportions of Orange and Red complex species (p<0.05) than CH sites from periodontally healthy subjects. Red complex species were positively associated with the expression of all biomarkers (p<0.05), while Purple and Yellow complex species had negative correlations with IL-1β and IL-8 (p<0.05). CH sites from periodontitis subjects present higher levels of GCF biomarkers and periodontal pathogens than CH sites from periodontally healthy subjects. Different microbial complexes demonstrated distinct associations with specific GCF biomarkers.
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通讯作者：
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作者：
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