Whole genome analysis of Japanese bovine toroviruses reveals natural recombination between porcine and bovine toroviruses.
Whole genome analysis of Japanese bovine toroviruses reveals natural recombination between porcine and bovine toroviruses.
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DOI：
10.1016/j.meegid.2015.12.013
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发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
Nagai M
中科院分区：
文献类型：
--
作者：
Ito M;Tsuchiaka S;Naoi Y;Otomaru K;Sato M;Masuda T;Haga K;Oka T;Yamasato H;Omatsu T;Sugimura S;Aoki H;Furuya T;Katayama Y;Oba M;Shirai J;Katayama K;Mizutani T;Nagai M
牛乳头状病毒（btov）属于冠状病毒科乳头状病毒亚科，是引起牛肠道疾病的病原体。在日本，btov分布在全国各地，引起小牛和奶牛的胃肠道感染。本研究测定了两种日本btov的全基因组序列，以及两种日本btov和一种来自日本偏远地区的猪圆环病毒（PToV）的部分基因组序列，并进行了遗传分析。双核苷酸比对和系统发育分析显示，日本BToV具有较高的同源性，与BToV Breda1在S、M和HE编码区具有较高的相似性。日本btov与猪torovirus在ORF1a、ORF1b和N编码区以及5 ‘和3 ’非翻译区表现出高度的相似性，提示存在自然重组事件。重组分析将假定的重组断点定位在ORF1b和HE区域的3 '端。这些结果表明，日本BToV的种间重组性质导致BToV Breda1与ptov的关系更为密切。鉴定了猪和牛环状病毒之间的重组事件。重组断点定位于ORF1b和HE编码区。这些重组病毒在日本很普遍。
Bovine toroviruses (BToVs), belong to the subfamily Toroviridae within the family Coronaviridae, and are pathogens, causing enteric disease in cattle. In Japan, BToVs are distributed throughout the country and cause gastrointestinal infection of calves and cows. In the present study, complete genome sequences of two Japanese BToVs and partial genome sequences of two Japanese BToVs and one porcine torovirus (PToV) from distant regions in Japan were determined and genetic analyses were performed. Pairwise nucleotide comparison and phylogenetic analyses revealed that Japanese BToVs shared high identity with each other and showed high similarities with BToV Breda1 strain in S, M, and HE coding regions. Japanese BToVs showed high similarities with porcine toroviruses in ORF1a, ORF1b, and N coding regions and the 5′ and 3′ untranslated regions, suggestive of a natural recombination event. Recombination analyses mapped the putative recombinant breakpoints to the 3′ ends of the ORF1b and HE regions. These findings suggest that the interspecies recombinant nature of Japanese BToVs resulted in a closer relationship between BToV Breda1 and PToVs. Recombination events between porcine and bovine torovirus were identified. Recombinant breakpoints were mapped at ORF1b and HE coding regions. These recombinant viruses are prevalent throughout Japan.
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影响因子：
5
作者：
Draker R;Roper RL;Petric M;Tellier R
通讯作者：
Tellier R
影响因子：
2.7
作者：
Aita, Tsunehiko;Kuwabara, Masaki;Tsunemitsu, Hiroshi
通讯作者：
Tsunemitsu, Hiroshi
影响因子：
1.5
作者：
Hoet, AE;Nielsen, PR;Saif, LJ
通讯作者：
Saif, LJ
影响因子：
0.9
作者：
Matiz, K;Kecskeméti, S;Nagy, B
通讯作者：
Nagy, B
影响因子：
1
作者：
Hoet, AE;Cho, KO;Saif, LJ
通讯作者：
Saif, LJ