Identification and characterization of An-4, a potential quantitative trait locus for awn development in rice.
Identification and characterization of An-4, a potential quantitative trait locus for awn development in rice.
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DOI：
10.1186/s12870-021-03055-w
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发表时间：
2021-06-29
影响因子：
5.3
通讯作者：
Li R
中科院分区：
文献类型：
--
作者：
Qin B;Lu T;Xu Y;Shen W;Liu F;Xie X;Li Y;Wang K;Li R
水稻芒是与产量性状密切相关的重要驯化性状。因此，鉴定水稻芒发育相关基因对阐明水稻芒发育的分子机制和产量性状的遗传改良具有重要意义。本研究以广西普通长芒野生稻（GXCWR，Oryza rufipogon Griff.）和短芒籼稻品种9311，鉴定了芒发育的一个潜在数量性状位点An-4。然后，An-4被精细定位到2号染色体的56 kb区域，其中包含4个注释基因。其中Os 02 g 0594800是An-4的潜在候选基因。An-4对芒的发育和几个产量性状表现出多效性。扫描电镜（SEM）分析表明，An-4在小穗发育的Sp 7和Sp 8期显著促进芒的发育。转录组分析表明，An-4可能通过调控与芒的生长发育过程、通道调控和胞外区相关基因的表达来影响芒的发育。与9311相比，CSSL 128中OsRR 5的表达水平显著下调，而OsCKX 2和OsGA 2 ox 5的表达水平显著上调。此外，我们的研究表明，An-4与其他芒发育基因，如An-1，An-2/LABA 1和An-3/GAD 1/RAE 2具有加性效应。An-4的鉴定为克隆An-4基因及进一步阐明芒发育的分子机制奠定了基础。此外，从9311中鉴定出的有利等位变异将有助于水稻产量性状的改良。在线版本包含补充材料，可通过10.1186/s12870-021-03055-w获得。
Awn of rice is an important domestication trait closely associated with yield traits. Therefore, the identification of genes for awn development is of great significance for the elucidation of molecular mechanism of awn development and the genetic improvement of yield traits in rice. In this study, using chromosome segment substitution lines (CSSLs) derived from a long-awned Guangxi common wild rice (GXCWR, Oryza rufipogon Griff.) and a short-awned indica cultivar 9311, we identified An-4, a potential quantitative trait locus (QTL) for awn development. Then, An-4 was fine mapped into a 56-kb region of chromosome 2, which contained four annotated genes. Among these four annotated genes, Os02g0594800 was concluded to be the potential candidate gene for An-4. An-4 exhibited pleiotropic effects on awn development and several yield traits. Scanning electron microscopy (SEM) analysis showed that An-4 significantly promoted awn development at Sp7 and Sp8 stage of spikelet development. Transcriptome analysis suggested that An-4 might influence the development of awn by regulating the expression of genes related to growth, developmental process, channel regulation and extracellular region. By contrast to those of 9311, the expression level of OsRR5 in CSSL128 was significantly down-regulated, whereas the expression levels of OsCKX2 and OsGA2ox5 in CSSL128 were significantly up-regulated. In addition, our study showed that An-4 had additive effects with other genes for awn development, such as An-1, An-2/LABA1 and An-3/GAD1/RAE2. The identification of An-4 lays a foundation for cloning of An-4 and further elucidation of the molecular mechanism of awn development. Moreover, the identification of favorable allelic variation of An-4 from 9311 will be useful to improve rice yield traits. The online version contains supplementary material available at 10.1186/s12870-021-03055-w.
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影响因子：
4.2
作者：
Sweeney M;McCouch S
通讯作者：
McCouch S
影响因子：
5.1
作者：
Khush, GS
通讯作者：
Khush, GS
影响因子：
5.4
作者：
Cai, HW;Morishima, H
通讯作者：
Morishima, H
影响因子：
3.3
作者：
Gu, XY;Kianian, SF;Foley, ME
通讯作者：
Foley, ME
DOI：
10.1534/g3.115.020834
发表时间：
2015-09-02
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Furuta T;Komeda N;Asano K;Uehara K;Gamuyao R;Angeles-Shim RB;Nagai K;Doi K;Wang DR;Yasui H;Yoshimura A;Wu J;McCouch SR;Ashikari M
通讯作者：
Ashikari M