MoSET1-dependent transcription factors regulate different stages of infection-related morphogenesis in Pyricularia oryzae
MoSET1-dependent transcription factors regulate different stages of infection-related morphogenesis in Pyricularia oryzae
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MoSET1依赖性转录因子调节稻瘟病菌感染相关形态发生的不同阶段
DOI：
10.1007/s10327-022-01111-3
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发表时间：
2023
影响因子：
1.2
通讯作者：
Hitoshi Nakayashiki
中科院分区：
文献类型：
--
作者：
Dang Ngoc Minh ; Yusaku Tsukahara ; Dang An Thach ; Ken-ich Ikeda ; Hitoshi Nakayashiki
稻瘟病菌的H3K4组蛋白甲基转移酶MoSET1在真菌感染相关的形态发生中起关键作用。我们之前鉴定了大约400个基因的表达可能在附着胞形成过程中直接受到MoSET1的调控。在这里，我们重点研究了在感染期间诱导的5个moset1依赖性转录因子（TF）基因，对5个基因中的3个（MGG_04699, MGG_06898, MGG_07450）采用基因缺失方法，对其余两个基因（MGG_00472和MGG_07386）采用基因沉默技术，因为难以构建基因缺失突变体。敲除突变体和敲除突变体的表型分析表明，MGG_06898对产孢至关重要，MGG_04699参与附着胞的形成，MGG_00472和MGG_04699是真菌完全毒力所必需的。这些结果表明，MoSET1通过控制转录因子，进一步调节p感染过程的不同步骤，从而参与真菌的致病性。oryzae。
MoSET1, an H3K4 histone methyltransferase inPyricularia oryzaeplays a key role in infection-related morphogenesis of the fungus. We previously identified approximately 400 genes for which expression might be regulated directly by MoSET1 during appressorium formation. Here, we focused on five MoSET1-dependent transcription factor (TF) genes that were induced during infection, using a gene deletion approach for three of the five genes (MGG_04699, MGG_06898, MGG_07450) and, for the remaining two genes (MGG_00472 and MGG_07386), a gene silencing technique due to difficulty in constructing a gene deletion mutant. Phenotypic characterization of the knockout and -down mutants revealed that MGG_06898 was crucial for sporulation, MGG_04699 was involved in appressorium formation, and MGG_00472 and MGG_04699 were required for full virulence of the fungus. These results demonstrated that MoSET1 contributes to the pathogenicity of the fungus by controlling transcription factors that further regulate different steps in the infection process ofP. oryzae.
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DOI：
--
发表时间：
1999
期刊：
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