Granulocyte collections: comparison of two apheresis systems
Granulocyte collections: comparison of two apheresis systems
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粒细胞采集：两种单采系统的比较
作者：
Thorausch K;Schulz M;Bialleck H;Luxembourg B;Seifried E;Bonig H
背景尽管缺乏明确的临床证据证明患者相关的结果得到改善，但供体粒细胞浓缩物仍常规用于粒细胞功能缺陷或短暂性中性粒细胞减少症以及细菌或真菌恶化（风险）的患者。通过单采术从健康供体中收集粒细胞，这些捐献者在单采术前一天晚上用粒细胞集落刺激因子和/或类固醇进行中性粒细胞动员，并在单采术期间使用羟乙基淀粉，以增强红细胞 (RBC) 的沉降，从而促进中性粒细胞的收集。 研究设计和 方法用标准单采设备进行粒细胞单采手术，包括用于外周血“干细胞”采集的常用单采系统 COBE Spectra MNC (Terumo BCT)，使用与 MNC 采集相同的管道组，但使用不同的软件协议 PMN。用于粒细胞采集的自动化单采系统 Spectra Optia IDL (Terumo BCT) 于 2011 年 10 月投入使用。从那时起，我们的站点已进行 70 次粒细胞单采手术，其中新旧系统各 35 次。结果单采手术自始至终都具有良好的耐受性。使用 Spectra Optia IDL 在除一个集合之外的所有集合中均实现了 1 × 1010 个中性粒细胞的目标剂量。 Spectra Optia IDL 采集效率提高约 20%。产品含有较多的非目标白细胞（单核细胞），但红细胞和血小板较少。虽然使用 Spectra Optia IDL 处理更少的血液即可达到相同的粒细胞剂量，但两种单采设备之间的临床相关差异并不明显。结论两种单采系统都能够产生类似的粒细胞浓缩物。
BackgroundDonor granulocyte concentrates are routinely administered to patients with granulocyte function defects or transient neutropenia and (risk of) bacterial or fungal exacerbations, despite lack of definitive clinical proof for patient‐relevant outcome improvement. Granulocytes are collected by apheresis from healthy donors treated with granulocyte–colony‐stimulating factor and/or steroids for neutrophil mobilization the evening before apheresis, as well as with hydroxyethyl starch during apheresis, to enhance sedimentation of red blood cells (RBCs) and thus to facilitate accessibility of neutrophils for collection.Study Design and MethodsGranulocyte apheresis procedures are performed with standard apheresis equipment, including with the frequently used apheresis system for peripheral blood “stem cell” collection, COBE Spectra MNC (Terumo BCT), using the same tubing set as for MNC collection, but a different software protocol, PMN. An automated apheresis system for granulocyte collection, Spectra Optia IDL (Terumo BCT), became available in October 2011. Since then, 70 granulocyte apheresis procedures have been performed at our site, 35 each with the new and old systems.ResultsApheresis procedures were well tolerated throughout. The target dose of 1 × 1010neutrophils was achieved in all but one collection with Spectra Optia IDL. Spectra Optia IDL collections were approximately 20% more efficient. Products contained more nontarget white blood cells (mononuclear cells), but fewer RBCs and platelets. Although less blood had to be processed with Spectra Optia IDL to achieve the same granulocyte dose, clinically relevant differences between the two apheresis devices were not apparent.ConclusionBoth apheresis systems are similarly capable of generating granulocyte concentrates.
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2.9
作者：
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影响因子：
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作者：
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影响因子：
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