Lipid Coverage in Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging of Mouse Lung Tissues.
Lipid Coverage in Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging of Mouse Lung Tissues.
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小鼠肺组织纳喷雾解吸电喷雾电离质谱成像中的脂质覆盖。
DOI：
10.1021/acs.analchem.9b02045
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发表时间：
2019-09-17
影响因子：
7.4
通讯作者：
Laskin, Julia
中科院分区：
文献类型：
--
作者：
Nguyen, Son N.;Kyle, Jennifer E.;Dautel, Sydney E.;Sontag, Ryan;Luders, Teresa;Corley, Richard;Ansong, Charles;Carson, James;Laskin, Julia
脂质是一组天然存在的分子，不仅有助于肺的结构完整性，防止肺泡萎陷，而且在抗炎反应和抗病毒保护中发挥重要作用。肺中脂质的类型和空间定位的改变在各种疾病中起着至关重要的作用，例如早产儿的呼吸窘迫综合征（RDS）和氧化应激影响的疾病，例如暴露于环境应激源后的肺炎、肺气肿和肺癌。准确测量肺组织中脂质和代谢物的空间分布的能力提供了与肺功能、发育和疾病状态相关的重要分子见解。纳米喷雾解吸电喷雾电离（nano-DESI）和其他环境电离质谱技术能够在最少甚至完全没有样品制备的情况下对处于其天然状态的复杂样品进行无标记成像。然而，在纳米-DESI质谱成像（MSI）实验中获得的脂质覆盖率以前没有被表征。在这项工作中，将小鼠肺组织的nano-DESI MSI中的脂质覆盖深度与使用两种不同程序制备的组织提取物的液相色谱串联质谱（LC-MS/MS）脂质组学分析进行比较：全肺样品的标准Folch提取方法和提取到nano-DESI MSI实验中使用的90%甲醇/10%水混合物中。正离子和负离子模式nano-DESI MSI的组合在小鼠肺组织中鉴定了20个脂质亚类和19种代谢物（总共284种）中的265种独特脂质。除了三酰甘油（TG）物质，nano-DESI MSI提供了与使用甲醇/水组织提取物进行的LC-MS/MS实验相当的覆盖率，与基于Folch提取的全肺脂质组学分析相比，覆盖率高达50%。这些结果证明了纳米DESI MSI对于组织切片中脂质的全面空间分辨分析的实用性。纳米DESI MSI和LC-MS/MS脂质组学的组合对于探索肺发育期间脂质分布的变化以及由疾病或暴露于环境毒物引起的变化特别有用。
Lipids are a naturally occurring group of molecules that not only contribute to the structural integrity of the lung preventing alveolar collapse but also play important roles in the anti-inflammatory responses and antiviral protection. Alteration in the type and spatial localization of lipids in the lung plays a crucial role in various diseases, such as respiratory distress syndrome (RDS) in preterm infants and oxidative stress-influenced diseases, such as pneumonia, emphysema, and lung cancer following exposure to environmental stressors. The ability to accurately measure spatial distributions of lipids and metabolites in lung tissues provides important molecular insights related to lung function, development, and disease states. Nanospray desorption electrospray ionization (nano-DESI) and other ambient ionization mass spectrometry techniques enable label-free imaging of complex samples in their native state with minimal to absolutely no sample preparation. However, lipid coverage obtained in nano-DESI mass spectrometry imaging (MSI) experiments has not been previously characterized. In this work, the depth of lipid coverage in nano-DESI MSI of mouse lung tissues was compared to liquid chromatography tandem mass spectrometry (LC-MS/MS) lipidomics analysis of tissue extracts prepared using two different procedures: standard Folch extraction method of the whole lung samples and extraction into a 90% methanol/10% water mixture used in nano-DESI MSI experiments. A combination of positive and negative ionization mode nano-DESI MSI identified 265 unique lipids across 20 lipids subclasses and 19 metabolites (284 in total) in mouse lung tissues. Except for triacylglycerols (TG) species, nano-DESI MSI provided comparable coverage to LC-MS/MS experiments performed using methanol/water tissue extracts and up to 50% coverage in comparison with the Folch extraction-based whole lung lipidomics analysis. These results demonstrate the utility of nano-DESI MSI for comprehensive spatially resolved analysis of lipids in tissue sections. A combination of nano-DESI MSI and LC-MS/MS lipidomics is particularly useful for exploring changes in lipid distributions during lung development, as well as resulting from disease or exposure to environmental toxicants.
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影响因子：
4.2
作者：
Bergman, Hilde-Marlene;Lundin, Erik;Lanekoff, Ingela
通讯作者：
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DOI：
10.1073/pnas.96.24.13995
发表时间：
1999-11-23
影响因子：
11.1
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影响因子：
3.3
作者：
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