Direct stacking of sequence-specific nuclease-induced mutations to produce high oleic and low linolenic soybean oil.
Direct stacking of sequence-specific nuclease-induced mutations to produce high oleic and low linolenic soybean oil.
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DOI：
10.1186/s12870-016-0906-1
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发表时间：
2016-10-13
影响因子：
5.3
通讯作者：
Zhang F
中科院分区：
文献类型：
--
作者：
Demorest ZL;Coffman A;Baltes NJ;Stoddard TJ;Clasen BM;Luo S;Retterath A;Yabandith A;Gamo ME;Bissen J;Mathis L;Voytas DF;Zhang F
调节大豆油中个别脂肪酸水平的能力有可能延长货架期和油炸稳定性，并改善营养特性。商品大豆油含有高水平的多不饱和亚油酸和亚麻酸，这会导致氧化不稳定--这个问题已经通过部分加氢解决了。然而，部分氢化会增加反式脂肪酸的水平，反式脂肪酸与心血管疾病有关。此前，我们培育了脂肪酸去饱和酶2-1A(FAD2-1A)和FAD2-1B基因敲除突变的大豆品系，导致油脂中单不饱和油酸(18：1)水平增加，亚油酸(18：2)和亚麻酸(18：3)水平降低。在这里，我们将FAD2-1A和FAD2-1B的突变与脂肪酸去饱和酶3A(FAD3A)的突变叠加在一起，以进一步降低亚麻酸的水平。通过将TALEN直接导入Fad2-1a、Fad2-1b大豆植株，将突变导入Fad3A。Fad2-1a Fad2-1b Fad3a植株的亚麻酸含量(2.5%)显著低于Fad2-1a Fad2-1b植株的亚麻酸含量(4.7%)。此外，油中的亚油酸水平明显较低(2.7%比5.1%)，油酸水平显著较高(82.2%比77.5%)。对转基因大豆Fad2-1a、Fad2-1b、Fad3a进行了鉴定。本文提出的方法为利用序列特异性核酸酶堆积大豆品质性状提供了一种有效的手段。最终产品的油酸水平高于80%，亚油酸和亚麻酸水平低于3%。本文的在线版本(doi：10.1186/s12870-0160906-1)包含补充材料，授权用户可以使用。
The ability to modulate levels of individual fatty acids within soybean oil has potential to increase shelf-life and frying stability and to improve nutritional characteristics. Commodity soybean oil contains high levels of polyunsaturated linoleic and linolenic acid, which contribute to oxidative instability – a problem that has been addressed through partial hydrogenation. However, partial hydrogenation increases levels of trans-fatty acids, which have been associated with cardiovascular disease. Previously, we generated soybean lines with knockout mutations within fatty acid desaturase 2-1A (FAD2-1A) and FAD2-1B genes, resulting in oil with increased levels of monounsaturated oleic acid (18:1) and decreased levels of linoleic (18:2) and linolenic acid (18:3). Here, we stack mutations within FAD2-1A and FAD2-1B with mutations in fatty acid desaturase 3A (FAD3A) to further decrease levels of linolenic acid. Mutations were introduced into FAD3A by directly delivering TALENs into fad2-1a fad2-1b soybean plants. Oil from fad2-1a fad2-1b fad3a plants had significantly lower levels of linolenic acid (2.5 %), as compared to fad2-1a fad2-1b plants (4.7 %). Furthermore, oil had significantly lower levels of linoleic acid (2.7 % compared to 5.1 %) and significantly higher levels of oleic acid (82.2 % compared to 77.5 %). Transgene-free fad2-1a fad2-1b fad3a soybean lines were identified. The methods presented here provide an efficient means for using sequence-specific nucleases to stack quality traits in soybean. The resulting product comprised oleic acid levels above 80 % and linoleic and linolenic acid levels below 3 %. The online version of this article (doi:10.1186/s12870-016-0906-1) contains supplementary material, which is available to authorized users.
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影响因子：
5.3
作者：
Pham AT;Lee JD;Shannon JG;Bilyeu KD
通讯作者：
Bilyeu KD
影响因子：
5.4
作者：
Anh-Tung Pham;Shannon, J. Grover;Bilyeu, Kristin D.
通讯作者：
Bilyeu, Kristin D.
影响因子：
2.3
作者：
Alt, JL;Fehr, WR;Sandhu, D
通讯作者：
Sandhu, D
影响因子：
13.8
作者：
Graef, George;LaVallee, Bradley J.;Clemente, Tom E.
通讯作者：
Clemente, Tom E.
影响因子：
2.3
作者：
Bilyeu, Kristin;Gillman, Jason D.;LeRoy, Allen R.
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LeRoy, Allen R.