Calretinin and calbindin architecture of the midline thalamus associated with prefrontal-hippocampal circuitry.
Calretinin and calbindin architecture of the midline thalamus associated with prefrontal-hippocampal circuitry.
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DOI：
10.1002/hipo.23271
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发表时间：
2021-07
期刊：
影响因子：
3.5
通讯作者：
Allen TA
中科院分区：
文献类型：
--
作者：
Viena TD;Rasch GE;Silva D;Allen TA
中线丘脑双向连接内侧前额叶皮层（mPFC）和海马（HC），形成一个独特的皮质-丘脑-皮质回路，对记忆和执行功能至关重要。虽然中线丘脑的解剖连接已被彻底调查，很少有人知道它的细胞组织内的每个核。在这里，我们使用免疫组织学技术，检查细胞分布在中线丘脑的基础上的钙结合蛋白小清蛋白（PV），钙视网膜蛋白（CR），钙结合蛋白（CB）。我们还研究了这些钙结合蛋白的reuniens细胞的人口中已知项目的mPFC和HC使用双荧光逆行腺相关病毒（AAV）为基础的跟踪方法。特别是，这些双重结合的mPFC-HC投射细胞被认为对于同步mPFC和HC活性是重要的。首先，我们证实了中线丘脑PV+神经元的缺乏。第二，我们发现CR+和CB+细胞在整个中线丘脑中具有共同的模式，CR+细胞沿沿着附近的第三脑室（3V）并穿透中线。CB+细胞始终更侧向和朝向中线丘脑的背腹侧范围的中间。值得注意的是，单标记CR+和CB+区部分重叠，包括双标记CR+/CB+细胞。在RE内，我们还观察到CR和CB亚区特异性多样性。有趣的是，RE中的双mPFC-HC投射神经元不表达检查的钙结合蛋白，但包含在CR+和CB+细胞的巢中。总体而言，中线丘脑被良好地组织成分布在整个区域中的CR+和CB+富集区，其中团聚体中的双mPFC-HC投射细胞代表独特的细胞群。这些结果提供了一个基于钙结合蛋白表达的中线丘脑细胞结构组织，并为将来这些不同细胞群在mPFC-HC相互作用中的功能作用的细胞类型特异性询问奠定了基础。
The midline thalamus bi-directionally connects the medial prefrontal cortex (mPFC) and hippocampus (HC) creating a unique cortico-thalamo-cortico circuit fundamental to memory and executive function. While the anatomical connectivity of midline thalamus has been thoroughly investigated, little is known about its cellular organization within each nucleus. Here we used immunohistological techniques to examine cellular distributions in the midline thalamus based on the calcium binding proteins parvalbumin (PV), calretinin (CR), and calbindin (CB). We also examined these calcium binding proteins in a population of reuniens cells known to project to both mPFC and HC using a dual fluorescence retrograde adenoassociated virus (AAV) based tracing approach. These dual reuniens mPFC-HC projecting cells, in particular, are thought to be important for synchronizing mPFC and HC activity. First, we confirmed the absence of PV+ neurons in the midline thalamus. Second, we found a common pattern of CR+ and CB+ cells throughout midline thalamus with CR+ cells running along the nearby third ventricle (3V) and penetrating the midline. CB+ cells were consistently more lateral and toward the middle of the dorsal-ventral extent of the midline thalamus. Notably, single-labeled CR+ and CB+ zones were partially overlapping and included dual-labeled CR+/CB+ cells. Within RE, we also observed a CR and CB subzone specific diversity. Interestingly, dual mPFC-HC projecting neurons in RE expressed none of the calcium binding proteins examined, but were contained in nests of CR+ and CB+ cells. Overall, the midline thalamus was well organized into CR+ and CB+ rich zones distributed throughout the region, with dual mPFC-HC projecting cells in reuniens representing a unique cell population. These results provide a cytoarchitectural organization in the midline thalamus based on calcium binding protein expression, and sets the stage for future cell-type specific interrogations of the functional role of these different cell populations in mPFC-HC interactions.
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影响因子：
3.8
作者：
ARAI, R;JACOBOWITZ, DM;DEURA, S
通讯作者：
DEURA, S
影响因子：
2.8
作者：
Csillik, B;Mihály, A;Fenyo, R
通讯作者：
Fenyo, R
DOI：
10.1111/bpa.12216
发表时间：
2015-09
期刊：
Brain pathology (Zurich, Switzerland)
影响因子：
--
作者：
Al-Mashhadi S;Simpson JE;Heath PR;Dickman M;Forster G;Matthews FE;Brayne C;Ince PG;Wharton SB;Medical Research Council Cognitive Function and Ageing Study
通讯作者：
Medical Research Council Cognitive Function and Ageing Study
影响因子：
3.4
作者：
Bokor, H;Csáki, A;Kiss, J
通讯作者：
Kiss, J
影响因子：
12.7
作者：
BRAAK, H;BRAAK, E
通讯作者：
BRAAK, E