Physiological and transcriptome analysis of He-Ne laser pretreated wheat seedlings in response to drought stress.
Physiological and transcriptome analysis of He-Ne laser pretreated wheat seedlings in response to drought stress.
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氦氖激光预处理小麦幼苗响应干旱胁迫的生理和转录组分析
DOI：
10.1038/s41598-017-06518-z
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发表时间：
2017-07-21
影响因子：
4.6
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Qiu Z;Yuan M;He Y;Li Y;Zhang L
干旱是世界范围内的一个严重问题，它降低了作物产量。激光在提高植物幼苗对各种非生物胁迫的耐受性方面具有积极的生理作用。然而，氦氖激光辐照诱导小麦适应干旱生理变化的分子机制尚不清楚。在这里，我们进行了大规模的转录组测序，以确定He-Ne激光预处理小麦幼苗在干旱胁迫下的分子作用。共鉴定出98.822个转录本，其中有820个转录本在干旱胁迫下He-Ne激光预处理小麦幼苗中与单独干旱胁迫下存在差异表达。与光合作用、养分吸收和转运、活性氧稳态控制和转录调控相关的代表性转录本在He-Ne激光预处理小麦幼苗中主要表达。由此可见，He-Ne激光预处理可能导致光合作用、抗氧化和渗透积累等生理过程的上调，从而提高了水稻的抗旱性。这些发现将扩大我们对激光照射小麦抗旱性相关复杂分子事件的理解，并为未来植物对环境胁迫适应性的研究提供丰富的遗传资源。
Drought stress is a serious problem worldwide that reduces crop productivity. The laser has been shown to play a positive physiological role in enhancing plant seedlings tolerance to various abiotic stresses. However, little information is available about the molecular mechanism of He-Ne laser irradiation induced physiological changes for wheat adapting to drought conditions. Here, we performed a large-scale transcriptome sequencing to determine the molecular roles of He-Ne laser pretreated wheat seedlings under drought stress. There were 98.822 transcripts identified, and, among them, 820 transcripts were found to be differentially expressed in He-Ne laser pretreated wheat seedlings under drought stress compared with drought stress alone. Furthermore, most representative transcripts related to photosynthesis, nutrient uptake and transport, homeostasis control of reactive oxygen species and transcriptional regulation were expressed predominantly in He-Ne laser pretreated wheat seedlings. Thus, the up-regulated physiological processes of photosynthesis, antioxidation and osmotic accumulation because of the modified expressions of the related genes could contribute to the enhanced drought tolerance induced by He-Ne laser pretreatment. These findings will expand our understanding of the complex molecular events associated with drought tolerance conferred by laser irradiation in wheat and provide abundant genetic resources for future studies on plant adaptability to environmental stresses.
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