Paradoxical effects of galloyl motifs in the interactions of proanthocyanidins with collagen-rich dentin.
Paradoxical effects of galloyl motifs in the interactions of proanthocyanidins with collagen-rich dentin.
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Galloyl基序在原核蛋白与富含胶原蛋白的牙本质的相互作用中的矛盾作用。
DOI：
10.1002/jbm.a.37276
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发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Bedran-Russo AK
中科院分区：
文献类型：
--
作者：
Alania Y;Zhou B;Reis M;Leme-Kraus AA;McAlpine JB;Chen SN;Pauli GF;Bedran-Russo AK
植物源性原花青素（PAC）介导的牙本质细胞外基质（ECM）的物理化学修饰。PAC的结构-活性关系在很大程度上仍然是未知的，主要是由于粗提取物的复杂组成的变化，以及纯化和机制评估的挑战。为了评估没食子酰化的PAC作为对牙本质ECM的高但不稳定的生物改性活性的重要贡献者的作用，我们通过酶促水解从三种富含没食子酰的PAC的提取物（茶树、葡萄属葡萄和弗吉尼亚金缕梅）中去除没食子酰部分（去没食子酰化）。牙本质的生物力学和生物学特性进行了评估后，这些提取物与他们的去没食子酸对应物的处理。发现所有提取物的牙本质基质的复合模量增加，然而，与去没食子酰化版本相比，粗提取物显著更高。在所研究的植物中表现出最高含量的没食子酰化PAC，茶树粗提物在孵育一个月后也表现出最大的机械性能恢复。去没食子酰化不改变牙本质ECM的阻尼能力。此外，PAC介导的保护蛋白水解降解不受去没食子酰化。去没食子酰化实验证实，没食子酰化的富PAC提取物中的没食子酸在牙本质ECM中驱动更强但显著更不持续的机械效应。
Plant-derived proanthocyanidins (PACs) mediate physicochemical modifications to the dentin extracellular matrix (ECM). The structure-activity relationships of PACs remain largely unknown, mostly due to the varied complex composition of crude extracts, as well as the challenges of purification and mechanistic assessment. To assess the role of galloylated PACs as significant contributors to high yet unstable biomodification activity to the dentin ECM, we removed the galloyl moieties (de-galloylation) via enzymatic hydrolysis from three galloyl-rich PAC-containing extracts (Camellia sinensis, Vitis vinifera, and Hamamelis virginiana). The biomechanical and biological properties of dentin were assessed upon treatment with these extracts vs. their de-galloylated counterparts. An increase in the complex modulus of the dentin matrix was found with all extracts, however, the crude extract was significantly higher when compared to the de-galloylated version. Exhibiting the highest content of galloylated PACs among the investigated plants, Camellia sinensis crude extract also exhibited the biggest relapse in mechanical properties after one-month incubation. De-galloylation did not modify the damping capacity of dentin ECM. Moreover, PAC-mediated protection against proteolytic degradation was unaffected by de-galloylation. The de-galloylation experiments confirmed that gallic acid in galloylated rich-PAC extracts drive a stronger yet significantly less sustained mechanical effect of in dentin ECM.
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影响因子：
5
作者：
Bedran-Russo, Ana K.;Pauli, Guido F.;Chen, Shao-Nong;McAlpine, James;Castellan, Carina S.;Phansalkar, Rasika S.;Aguiar, Thaiane R.;Vidal, Cristina M. P.;Napotilano, Jose G.;Nam, Joo-Won;Leme, Ariene A.
通讯作者：
Leme, Ariene A.
DOI：
10.1016/j.dental.2020.09.014
发表时间：
2020-12
期刊：
Dental materials : official publication of the Academy of Dental Materials
影响因子：
--
作者：
Alania Y;Reis MCD;Nam JW;Phansalkar RS;McAlpine J;Chen SN;Pauli GF;Bedran-Russo AK
通讯作者：
Bedran-Russo AK
影响因子：
5
作者：
Silva Sousa, Ana Beatriz;Vidal, Cristina M. P.;Bedran-Russo, Ana K.
通讯作者：
Bedran-Russo, Ana K.
影响因子：
5
作者：
Tezvergil-Mutluay, Arzu;Agee, Kelli A.;Hoshika, Tomohiro;Carrilho, Marcela;Breschi, Lorenzo;Tjaderhane, Leo;Nishitani, Yoshihiro;Carvalho, Ricardo M.;Looney, Stephen;Tay, Franklin R.;Pashley, David H.
通讯作者：
Pashley, David H.
影响因子：
6.1
作者：
Li M;Hagerman AE
通讯作者：
Hagerman AE