The correlation between IGF-II and Bcl-2 expression in colorectal adenocarcinoma
The correlation between IGF-II and Bcl-2 expression in colorectal adenocarcinoma
复制标题
结直肠腺癌中IGF-II与Bcl-2表达的相关性
DOI：
10.1007/s12032-011-9881-4
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发表时间：
2012-06
期刊：
影响因子：
--
通讯作者：
陈元
中科院分区：
文献类型：
--
作者：
张明生;胡爱华;邱红;熊慧华;陈元
本研究旨在探讨IGF-II和Bcl-2在结直肠腺癌中的表达及其与结直肠腺癌转移的关系。选取60例结直肠腺癌石蜡包埋标本，15例正常结直肠组织作为对照。原位杂交检测IGF-Ⅱ mRNA表达，免疫组化检测Bcl-2沿着增殖细胞核抗原（PCNA）蛋白表达。TUNEL法检测细胞凋亡。阳性细胞比率小于30%的标本定义为阴性。结直肠腺癌组织中IGF-II mRNA和Bcl-2蛋白的表达水平（分别为39.64 ± 7.38%和30.74 ± 7.22%）明显高于正常结直肠组织（分别为22.56 ± 4.21%和12.17 ± 1.94%）（P< 0.01）。其水平与Dukes分期和淋巴结转移有关，而与患者年龄、性别、肿瘤部位、肿瘤大小和肿瘤分化程度无关。Dukes分期中IGF-Ⅱ mRNA表达与Bcl-2蛋白表达呈负相关（P< 0.05）。IGF-II mRNA表达与PCNA、细胞凋亡呈正相关（P <0.01），与Bcl-2表达呈负相关（P< 0.01）。Bcl-2与PCNA表达无相关性（P> 0.05）。IGF-II mRNA（+）和Bcl-2（-）的患者预后最差。IGF-II和Bcl-2的表达与结直肠腺癌的临床表现有关，因此，IGF-II和Bcl-2的检测对结直肠腺癌的诊断和预后有重要意义。
Our aim for this study was to investigate the correlation and clinical significance between the expression of IGF-II and Bcl-2 in colorectal adenocarcinoma, especially in terms of the metastasis of colorectal adenocarcinoma. Sixty paraffin embedded samples of colorectal adenocarcinoma were selected, and fifteen normal colorectal tissues were used as controls. IGF-II mRNA was detected using in situ hybridization, and the expression of Bcl-2 along with the proliferating cell nuclear antigen (PCNA) protein was detected through immunohistochemistry. The TUNEL assay was used to detect apoptosis. Specimens with a positive cell ratio less than 30% were defined as negative. The levels of IGF-II mRNA and the Bcl-2 protein were significantly higher in colorectal adenocarcinoma (39.64 ± 7.38% and 30.74 ± 7.22%, respectively) than in normal colorectal tissues (22.56 ± 4.21% and 12.17 ± 1.94%, respectively) (P< 0.01). The levels were related to Dukes′ stage and lymph node metastases, but were unrelated to patient age, gender, tumor site, tumor size, and tumor differentiation. Also, a negative correlation was observed between IGF-II mRNA and Bcl-2 protein (P< 0.05) during Dukes′ stages. In addition, a positive correlation between IGF-II mRNA and PCNA or apoptosis, as well as a negative correlation between Bcl-2 and apoptosis were observed (P< 0.01). There was no correlation between Bcl-2 and PCNA (P> 0.05). The patients detected as IGF-II mRNA (+) and Bcl-2 (−) showed the worst prognosis. The expression of IGF-II and Bcl-2 was correlated with the clinical manifestation of colorectal adenocarcinoma; thus, the assessment of both IGF-II and Bcl-2’s status will provide important information regarding the diagnosis and prognosis of colorectal adenocarcinoma.
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影响因子：
56.9
作者：
TSUJIMOTO, Y;FINGER, LR;CROCE, CM
通讯作者：
CROCE, CM
影响因子：
64.8
作者：
Geballe, TR;Oka, T
通讯作者：
Oka, T
影响因子：
3.5
作者：
Kawamoto, K;Onodera, H;Imamura, M
通讯作者：
Imamura, M
DOI：
10.1016/0006-291x(87)91635-4
发表时间：
1987-11
影响因子：
3.1
作者：
Quynh T. Rohlik;David J. Adams;Frederick C. Kull;Steven Jacobs
通讯作者：
Quynh T. Rohlik;David J. Adams;Frederick C. Kull;Steven Jacobs
影响因子：
39.2
作者：
LEROITH, D;BASERGA, R;ROBERTS, CT
通讯作者：
ROBERTS, CT