Isolation and Identification of Subgroup J Avian Leukosis Virus Inducing Multiple Systemic Tumors in Parental Meat-Type Chickens.
Isolation and Identification of Subgroup J Avian Leukosis Virus Inducing Multiple Systemic Tumors in Parental Meat-Type Chickens.
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诱发亲代肉鸡多系统肿瘤的J亚型禽白血病病毒的分离与鉴定
DOI：
10.3389/fvets.2020.614854
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发表时间：
2020
影响因子：
3.2
通讯作者：
Li C
中科院分区：
文献类型：
--
作者：
Cui N;Cui X;Huang Q;Yang S;Su S;Xu C;Li J;Li W;Li C
禽白血病病毒（ALV）不断进化以获得新的基因组特征来增强其致病性。本研究从20周龄以前达到麻痹症状的肉种鸡中分离到一株ALV-J毒株LH 20180301。剖检鸡显示皮下和肌肉出血，并在多个器官（包括骨、肝、脾和肾）中发生肿瘤。本研究通过对原病毒全序列的克隆和测序，探讨了该病毒的分子特征和致瘤性。已报道的ALV-J株LH 20180301的基因组结构与其他ALV高度保守。在病毒基因组中鉴定了病毒与内源性病毒之间的分离事件。与ALV-J原株HPRS-103相比，病毒基因组与ev-1的重组位点主要位于5′ UTR-gag和3′ UTR区。群特异性抗原gp 85编码蛋白的系统进化分析表明，LH 20180301与我国南方地方鸡种“黄鸡”中流行的ALV-J存在分支关系。在2012年以前的ALV-J分离株中，gp 85的9个氨基酸（N58，D 60，K70，A71，K108，N112，N113，N121，R272）高度保守，但在包括LH 20180301在内的晚期分离株中发现了多种突变。另外，LH 20180301株也具有与它们相同的3′ UTR缺失模式。因此，LH 20180301可能与这些病毒具有相同的祖先，可能是中国ALV-J进化的趋势。在ALV-J的gp 85和3′ UTR区发现的新的基因组特征可能为ALV-J的致瘤性增强提供了分子基础。
Avian leukosis virus (ALV) continues evolving to obtain new genomic characters to enhance its pathogenicity. In the present study, an ALV-J strain LH20180301 was isolated from broiler breeder chickens that reached the speak of paralyzation before 20-week-old. The necropsy chickens showed subcutaneous and muscular hemorrhage, and developed tumors in multiple organs including bone, liver, spleen, and kidney. The complete provirus was then cloned and sequenced to investigate the molecular characteristics and oncogenicity etiology of this virus associated with the outbreak of disease. The genomic structure of the reported ALV-J strain LH20180301 was highly conservative with other ALVs. Recombination events between the virus with endogenous virus were identified in the viral genome. Compared with the ALV-J original HPRS-103 strain, the major recombination sites of the viral genome with ev-1 were located in 5′ UTR-gag and 3′ UTR regions. Phylogenetic analysis of group specific antigen gp85 encoding protein showed that the LH20180301 branched with ALV-J prevalent in “yellow chickens” of local breeds in South China. Nine amino acids (N58, D60, K70, A71, K108, N112, N113, N121, R272) in the gp85 were highly conserved among ALV-J isolates before 2012, but various mutations were found in the late isolates including LH20180301. In addition, the LH20180301 strain also had the same deletion pattern of 3′ UTR with them. Therefore, LH20180301 might derive from the same ancestor with those viruses and may be the trend of ALV-J evolution in China. The defined new genomic characters in the gp85 and 3′ UTR region of ALV-J might provide the molecular basis for its enhanced oncogenicity.
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影响因子：
4.8
作者：
Shi M;Tian M;Liu C;Zhao Y;Lin Y;Zou N;Liu P;Huang Y
通讯作者：
Huang Y
影响因子：
3.8
作者：
PAYNE, LN;BROWN, SR;THOULESS, ME
通讯作者：
THOULESS, ME
影响因子：
1.4
作者：
Cui, Zhizhong;Sun, Shuhong;Wang, Jianxin
通讯作者：
Wang, Jianxin
DOI：
10.1136/vr.104626
发表时间：
2018-01-06
期刊：
The Veterinary record
影响因子：
--
作者：
Smith, Lorraine P;Petheridge, Lawrence;Welchman, David
通讯作者：
Welchman, David
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M