Omics profiles of fecal and oral microbiota change in irritable bowel syndrome patients with diarrhea and symptom exacerbation.
Omics profiles of fecal and oral microbiota change in irritable bowel syndrome patients with diarrhea and symptom exacerbation.
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DOI：
10.1007/s00535-022-01888-2
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发表时间：
2022-10
影响因子：
6.3
通讯作者：
Fukudo, Shin
中科院分区：
文献类型：
--
作者：
Tanaka, Yukari;Yamashita, Riu;Kawashima, Junko;Mori, Hiroshi;Kurokawa, Ken;Fukuda, Shinji;Gotoh, Yasuhiro;Nakamura, Keiji;Hayashi, Tetsuya;Kasahara, Yoshiyuki;Sato, Yukuto;Fukudo, Shin
肠易激综合征（IBS）是一种肠-脑相互作用的疾病，包括下丘脑-垂体-肾上腺轴失调和唾液皮质醇变化。然而，肠道微生物群在IBS症状加重期间的作用仍不清楚。我们测试了这样一个假设，即在IBS症状加重期间，粪便和口腔样本的微生物种类、基因转录物和化学成分发生了改变。粪便，唾液，和牙菌斑样品收集在基线从43名男子与IBS腹泻（IBS-D）和40名健康对照（HC）男子。IBS-D患者的样本也在症状加重期间采集。粪便微生物群的组成通过分析16 S rRNA基因、基于RNA的元转录组和来自有和无症状加重的HC和IBS患者的样品中的代谢物来确定。还使用组学方法分析了口腔样品。IBS症状加重期间的粪便微生物群与症状未加重时排便期间的粪便样本相比，在促炎模式和短链脂肪酸方面表现出显著差异。虽然HC和IBS-D患者之间的粪便微生物群丰度的致突变模式没有显著差异，但在与丁酸盐产生和神经内分泌激素（包括色氨酸-5-羟色胺-褪黑激素合成和谷氨酰胺/GABA）相关的细菌转录组的表达模式中检测到显著差异。HC和IBS-D患者在正常排便期间的菌斑微生物群组成不同。我们的研究结果表明，IBS-D患者在症状加重期间结肠宿主-微生物相互作用发生改变。粪便和口腔微生物组之间没有重叠。在线版本包含补充材料，可通过10.1007/s 00535 -022-01888-2获得。
Irritable bowel syndrome (IBS) is a disorder of gut–brain interaction, including dysregulation of the hypothalamic–pituitary–adrenal axis with salivary cortisol changes. However, the role of gastrointestinal microbiota during IBS symptom exacerbation remains unclear. We tested the hypothesis that the microbial species, gene transcripts, and chemical composition of fecal and oral samples are altered during the exacerbation of IBS symptoms. Fecal, salivary, and dental plaque samples were collected at baseline from 43 men with IBS with diarrhea (IBS-D) and 40 healthy control (HC) men. Samples in the IBS-D patients were also collected during symptom exacerbation. The composition of the fecal microbiota was determined by analyzing the 16S rRNA gene, RNA-based metatranscriptome, and metabolites in samples from HC and IBS patients with and without symptom exacerbation. Oral samples were also analyzed using omics approaches. The fecal microbiota during IBS symptom exacerbation exhibited significant differences in the phylogenic pattern and short-chain fatty acid compared with fecal samples during defecation when symptoms were not exacerbated. Although there were no significant differences in the phylogenic pattern of fecal microbiota abundance between HCs and IBS-D patients, significant differences were detected in the expression patterns of bacterial transcriptomes related to butyrate production and neuroendocrine hormones, including tryptophan-serotonin-melatonin synthesis and glutamine/GABA. The composition of plaque microbiota was different between HC and IBS-D patients during normal defecation. Our findings suggest that colonic host-microbial interactions are altered in IBS-D patients during exacerbation of symptoms. There were no overlaps between feces and oral microbiomes. The online version contains supplementary material available at 10.1007/s00535-022-01888-2.
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DOI：
10.1126/science.aan4526
发表时间：
2017-10-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Suda W;Luo C;Kawaguchi T;Motoo I;Narushima S;Kiguchi Y;Yasuma K;Watanabe E;Tanoue T;Thaiss CA;Sato M;Toyooka K;Said HS;Yamagami H;Rice SA;Gevers D;Johnson RC;Segre JA;Chen K;Kolls JK;Elinav E;Morita H;Xavier RJ;Hattori M;Honda K
通讯作者：
Honda K
影响因子：
5.2
作者：
Mazzoli R;Pessione E
通讯作者：
Pessione E
DOI：
10.1038/nri.2016.88
发表时间：
2016-10
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
Johansson ME;Hansson GC
通讯作者：
Hansson GC
影响因子：
7.6
作者：
Lu, WZ;Gwee, KA;Ho, KY
通讯作者：
Ho, KY
影响因子：
3.7
作者：
Holodniy, Mark;Brown, Sheldon T.;Darbyshire, Janet
通讯作者：
Darbyshire, Janet