Three-photon luminescence of gold nanorods and its applications for high contrast tissue and deep in vivo brain imaging.
Three-photon luminescence of gold nanorods and its applications for high contrast tissue and deep in vivo brain imaging.
复制标题
金纳米棒的三光子发光及其在高对比度组织和深部体内脑成像中的应用
作者：
Wang S;Xi W;Cai F;Zhao X;Xu Z;Qian J;He S
金纳米颗粒可用作生物成像应用的造影剂。本文研究了金纳米棒在飞秒激光激发下的多光子发光。用聚乙二醇（PEG）分子功能化的GNRs具有高的化学和光学稳定性，并且可以用作活体动物的深度体内成像的多光子发光纳米探针。我们已经发现，体内成像的深度取决于与GNRs相互作用的激发光的透射和聚焦能力。我们的研究重点是比较MPL从GNRs与两种不同的纵横比，以及他们的离体和在体成像效果下760 nm和1000 nm的激发，分别。这两个波长都位于生物组织的光学透明窗口（700-1000 nm）。PEG化GNRs通过尾静脉静脉注射到小鼠体内，并在主要器官和肿瘤组织中积累，在1000 nm fs激光照射后，由于明显的三光子发光（3 PL）信号，显示出高的图像对比度。在活体小鼠脑成像方面，GNRs在1000 nm fs激发下的3 PL成像深度可达600 μm，比GNRs在760 nm fs激发下的2 PL成像深度约深170 μm。
Gold nanoparticles can be used as contrast agents for bio-imaging applications. Here we studied multi-photon luminescence (MPL) of gold nanorods (GNRs), under the excitation of femtosecond (fs) lasers. GNRs functionalized with polyethylene glycol (PEG) molecules have high chemical and optical stability, and can be used as multi-photon luminescent nanoprobes for deep in vivo imaging of live animals. We have found that the depth of in vivo imaging is dependent upon the transmission and focal capability of the excitation light interacting with the GNRs. Our study focused on the comparison of MPL from GNRs with two different aspect ratios, as well as their ex vivo and in vivo imaging effects under 760 nm and 1000 nm excitation, respectively. Both of these wavelengths were located at an optically transparent window of biological tissue (700-1000 nm). PEGylated GNRs, which were intravenously injected into mice via the tail vein and accumulated in major organs and tumor tissue, showed high image contrast due to distinct three-photon luminescence (3PL) signals upon irradiation of a 1000 nm fs laser. Concerning in vivo mouse brain imaging, the 3PL imaging depth of GNRs under 1000 nm fs excitation could reach 600 μm, which was approximately 170 μm deeper than the two-photon luminescence (2PL) imaging depth of GNRs with a fs excitation of 760 nm.
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