Rice gall dwarf virus exploits tubules to facilitate viral spread among cultured insect vector cells derived from leafhopper Recilia dorsalis.
Rice gall dwarf virus exploits tubules to facilitate viral spread among cultured insect vector cells derived from leafhopper Recilia dorsalis.
复制标题
稻瘿矮化病毒利用小管促进病毒在源自叶蝉 Recilia dorsalis 的培养昆虫载体细胞中传播。
DOI：
10.3389/fmicb.2013.00206
复制
发表时间：
2013
影响因子：
5.2
通讯作者：
Wei T
中科院分区：
文献类型：
--
作者：
Chen H;Zheng L;Jia D;Zhang P;Chen Q;Liu Q;Wei T
呼肠孤病毒科水稻瘤矮缩病毒是呼肠孤病毒科的成员之一，在中国南部稻田反复流行。从广东省(中国南部)采集的一个RGDV分离物主要通过叶蝉介体背叶蝉以持续繁殖的方式传播。RGDV的感染诱导植物寄主和昆虫载体中形成含病毒的小管。在这项研究中，我们建立了背叶蝉的连续细胞培养，以研究这些微管在昆虫媒介中的功能作用。细胞病理学研究表明，含有病毒非结构蛋白Pns11和含有病毒颗粒的小管能够从培养的叶蝉细胞表面伸出。在杆状病毒系统中表达Pns11后，在非寄主昆虫细胞中形成了小管样结构，这表明Pns11是RGDV感染诱导小管形成所需的最小病毒因子。在培养的叶蝉细胞中，由Pns11基因合成的dsRNA诱导的RNA干扰抑制了Pns11的表达，消除了这种小管的形成，防止了RGDV在细胞之间的直接传播，而对病毒的增殖没有显著影响。所有这些结果表明，RGDV利用含病毒的小管促进病毒在其昆虫载体细胞中传播。
Rice gall dwarf virus (RGDV), a member of the family Reoviridae, causes repeated epidemics in rice fields in southern China. An RGDV isolate collected from Guangdong Province (southern China) is mainly transmitted by leafhopper vector Recilia dorsalis in a persistent-propagative manner. The infection by RGDV induces the formation of virus-containing tubules in the plant host and insect vector. In this study, we established continuous cell cultures of the leafhopper R. dorsalis to investigate the functional role of these tubules within the insect vector. Cytopathologic studies revealed that the tubules, which comprised viral non-structural protein Pns11 and contained viral particles, were able to protrude from the surface of cultured leafhopper cells. Tubule-like structures formed in non-host insect cells after the expression of Pns11 in a baculovirus system, suggesting that Pns11 was the minimal viral factor required for the formation of the tubules induced by RGDV infection. In cultured leafhopper cells, knockdown of Pns11 expression from RNA interference, induced by synthesized dsRNA from the Pns11 gene, abolished the formation of such tubules, preventing the direct cell-to-cell spread of RGDV without significant effects on viral multiplication. All these results show that RGDV exploits virus-containing tubules to facilitate viral spread among its insect vector cells.
登录
查看更多内容
影响因子：
3.7
作者：
FUKUSHI, T;KIMURA, I;SHIKATA, E
通讯作者：
SHIKATA, E
影响因子：
2.7
作者：
Moriyasu, Y.;Maruyama-Funatsuki, W.;Omura, T.
通讯作者：
Omura, T.
影响因子：
3.8
作者：
OMURA, T;MINOBE, Y;SAITO, Y
通讯作者：
SAITO, Y
影响因子：
5.4
作者：
Wei, Taiyun;Uehara-Ichiki, Tamaki;Omura, Toshihiro
通讯作者：
Omura, Toshihiro
影响因子：
2.7
作者：
Zhang, Heng-Mu;Xin, Xing;Adams, M. J.
通讯作者：
Adams, M. J.