Subfractionation of malignant variants of metastatic murine lymphosarcoma cells by countercurrent distribution in two-polymer aqueous phases.

Subfractionation of malignant variants of metastatic murine lymphosarcoma cells by countercurrent distribution in two-polymer aqueous phases.
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通过在两种聚合物水相中逆流分布对转移性鼠淋巴肉瘤细胞的恶性变体进行细分。

DOI:
10.1021/bi00524a052
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发表时间:
1981
期刊:
影响因子:
2.9
通讯作者:
Nicolson,GL
Nicolson,GL
中科院分区:
生物学3区
文献类型:
--
作者:
Miner,KM;Walter,H;Nicolson,GL

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Karen M. Miner, Harry Walter, and Garth L. Nicolson* abstract: The low metastatic murine lymphosarcoma pa-rental cell line RAW117-P and a high metastatic subline (RAW117-H10), selected sequentially 10 times for liver co-lonization, were grown in suspension and subjected to coun-tercurrent distribution (CCD) in a dextran-poly (ethylene glycol) aqueous phase system having an electrostatic potential difference between the phases. Both the RAW117-P and the RAW117-H10 sublines gave broad distribution curves, with the mean partition coefficient of RAW117-H10 cells being higher than the mean partition coefficient of RAW117-P cells.RAW117-P cells were kept in culture over a 2-month period during which time the mean partition coefficients increased. Concomitant with their in vivo drift in partition coefficients, the RAW 117-P cells also displayed increasing heterogeneity as evidenced by the appearance of broader and even multi-peaked CCD curves. However, the mean partition coefficients always remained lower than that of the RAW117-H10 subline. Cells from different cavities along the extraction train following
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