The Presence of Circulating Nucleated Red Blood Cells Is Associated With Disease Severity in Patients of Hemorrhagic Fever With Renal Syndrome.
The Presence of Circulating Nucleated Red Blood Cells Is Associated With Disease Severity in Patients of Hemorrhagic Fever With Renal Syndrome.
复制标题
循环有核红细胞的存在与肾综合征出血热患者的疾病严重程度相关
DOI：
10.3389/fmed.2021.665410
复制
发表时间：
2021
影响因子：
3.9
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Zhang J;Tang K;Zhang Y;Ma Y;Zhang C;Hu H;Jia X;Zhuang R;Jin B;Wang M;Zhang X;Liu D;Zhang Y
肾综合征出血热（HFRS）是由汉滩病毒（HTNV）引起的一种具有流行潜力的区域性传染病。红细胞（RBC）是外周血的主要成分。然而，红细胞的病理变化和HTNV感染过程中的潜在机制仍不清楚。因此，本研究旨在探讨HFRS患者外周血红细胞的变化。我们从HFRS患者中分离PBMCs并进行单细胞RNA测序。结果表明，HFRS患者外周血中的红细胞群可根据其细胞成分、基因表达谱和细胞表面标志物等特征被归类为有核红细胞（NRBC）。外周血NRBC计数越高，病情越重。此外，对与红细胞相关的血液学指标进行了分析，结果表明叶酸途径的损伤可能是NRBC存在的原因。这项研究首次表明HFRS患者外周血中NRBC的存在与疾病严重程度相关。这也是第一项研究表明HTNV病毒感染阻碍了RBC的成熟。因此，这项工作提供了进一步的见解，在HTNV感染过程中红细胞的作用和病理变化。
Hemorrhagic fever with renal syndrome (HFRS) is a regional infectious disease of epidemic potential caused by the Hantaan virus (HTNV). Red blood cells (RBCs) are the major components of peripheral blood. However, pathological changes in RBCs and the underlying mechanisms during HTNV infection remain largely unclear. Therefore, this study sought to explore changes in RBCs in the peripheral blood of HFRS patients. We isolated PBMCs from HFRS patients and performed single-cell RNA sequencing. The results showed that clusters of RBCs in the peripheral blood of HFRS could be classified as nucleated red blood cells (NRBC) based on their cellular components, gene expression profiles and cell surface markers. In addition, it was shown that the higher the count of NRBC in peripheral blood, the more severe the disease status was. Moreover, hematological indices related to RBCs were analyzed and the results showed that impairment in the folate pathway might be the possible reason behind the presence of NRBCs. This study, for the first time showed that the presence of NRBCs in the peripheral blood of HFRS patients was associated with disease severity. This was also the first study to show that infection with the HTNV virus hindered the maturation of RBCs. Therefore, this work provides further insights on the role of and pathological changes in RBCs during HTNV infection.
登录
查看更多内容
影响因子：
13.6
作者：
Babitt, Jodie L.;Lin, Herbert Y.
通讯作者：
Lin, Herbert Y.
影响因子：
20.3
作者：
Kautz, Leon;Nemeth, Elizabeta
通讯作者：
Nemeth, Elizabeta
DOI：
10.1001/jama.1954.02940500019007
发表时间：
1954-01-01
影响因子：
120.7
作者：
SCHWARTZ, SO;STANSBURY, F
通讯作者：
STANSBURY, F
影响因子：
64.8
作者：
Elahi, Shokrollah;Ertelt, James M.;Kinder, Jeremy M.;Jiang, Tony T.;Zhang, Xuzhe;Xin, Lijun;Chaturvedi, Vandana;Strong, Beverly S.;Qualls, Joseph E.;Steinbrecher, Kris A.;Kalfa, Theodosia A.;Shaaban, Aimen F.;Way, Sing Sing
通讯作者：
Way, Sing Sing
影响因子：
4.6
作者：
Xie, Ming;Dong, Yanying;Lv, Shemin
通讯作者：
Lv, Shemin