Feline Stool-Associated Circular DNA Virus (FeSCV) in Diarrheic Cats in China.
Feline Stool-Associated Circular DNA Virus (FeSCV) in Diarrheic Cats in China.
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中国腹泻猫中的猫粪便相关环状 DNA 病毒 (FeSCV)
DOI：
10.3389/fvets.2021.694089
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发表时间：
2021
影响因子：
3.2
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Hao X;Li Y;Hu X;Fu X;Dong J;Zhang H;Zhou P;Li S
猫粪便相关环状 DNA 病毒 (FeSCV) 是一种未分类的环状复制相关蛋白编码单链 (CRESS) DNA 病毒，于 2018 年在日本猫中发现。关于 FeSCV 的基因组特征和患病率的研究很少。为了调查FeSCV是否已在中国广东的家猫中传播，2018年在一家动物医院采集了腹泻猫的粪便样本，以促进FeSCV的研究。通过PCR扩增和测序获得FeSCV基因组，检测到的病毒被命名为PY4（GenBank号MT732515）。 PY4的基因组大小为2,034 nt，比报道的日本FeSCV毒株（KU7、KU8、KU9、KU14）的基因组（2,046 nt）小12 nt。 PY4株与日本FeSCV株有95.1～95.5%的同源性。值得注意的是，PY4的Cap蛋白在15个氨基酸位点发生突变，并且PY4基因组含有独特的开放阅读框3。此外，PY4的茎环结构中存在两个额外的碱基插入，间隔区的核苷酸同源性不高。基于 Rep 蛋白的系统发育树显示，PY4、日本 FeSCV 和啮齿动物粪便相关圆形病毒 (RodSCV) 聚集在一起，表明它们在系统发育进化中可能具有相似的起源。本研究对2018年在中国广州采集的样本进行病原学调查，20%（2/10）的样本呈FeSCV阳性。 PY4的ORF、茎环结构、Cap蛋白和基因间区序列与日本报道的有显着差异。这是中国首次报道家猫腹泻中出现FeSCV，迫切需要进一步的流行病学研究来评估该病毒对猫的影响。
Feline stool-associated circular DNA virus (FeSCV) is an unclassified circular replication-associated protein-encoding single-stranded (CRESS) DNA virus that was discovered in cats in Japan in 2018. Few studies on the genomic characteristics and prevalence of FeSCV have been conducted. To investigate whether FeSCV has been circulating in domestic cats in Guangdong, China, fecal samples were collected from cats with diarrhea in an animal hospital in 2018 to promote research on FeSCV. The FeSCV genome was obtained by PCR amplification and sequencing, and the detected virus was named PY4 (GenBank No. MT732515). The genome of PY4 was 2,034 nt in size, which was 12 nt smaller than the reported genome of Japanese FeSCV strains (KU7, KU8, KU9, KU14) (2,046 nt). The PY4 strain shared 95.1 ~ 95.5% homology with Japanese FeSCV strains. Notably, the Cap protein of PY4 was mutated at 15 amino acid sites, and the PY4 genome contained a unique open reading frame 3. In addition, there were two additional base insertions in the stem-loop structure of PY4, and the nucleotide homology of the spacer region was not high. A phylogenetic tree based on Rep proteins showed that PY4, Japanese FeSCVs and rodent stool-associated circular viruses (RodSCVs) clustered together, suggesting that they might share a similar origin in their phylogenetic evolution. In this study, samples collected in Guangzhou, China, in 2018 were subjected to an etiological investigation, and 20% (2/10) of the samples were positive for FeSCV. The ORFs, stem-loop structures, Cap proteins and intergenic region sequences of PY4 were significantly different from those reported in Japan. This is the first report of FeSCV in domestic cats with diarrhea in China, and further epidemiological studies are urgently needed to assess the impact of the virus on cats.
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影响因子：
6.4
作者：
Tan le V;van Doorn HR;Nghia HD;Chau TT;Tu le TP;de Vries M;Canuti M;Deijs M;Jebbink MF;Baker S;Bryant JE;Tham NT;BKrong NT;Boni MF;Loi TQ;Phuong le T;Verhoeven JT;Crusat M;Jeeninga RE;Schultsz C;Chau NV;Hien TT;van der Hoek L;Farrar J;de Jong MD
通讯作者：
de Jong MD
影响因子：
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通讯作者：
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影响因子：
16.6
作者：
Kinsella, Cormac M.;Bart, Aldert;Van der Hoek, Lia
通讯作者：
Van der Hoek, Lia
影响因子：
3.3
作者：
Mankertz, A;Çaliskan, R;Finsterbusch, T
通讯作者：
Finsterbusch, T
野生啮齿动物的粪便病毒植物。
影响因子：
6.7
作者：
Phan TG;Kapusinszky B;Wang C;Rose RK;Lipton HL;Delwart EL
通讯作者：
Delwart EL