Physical Properties and Antimicrobial Release Ability of Gentamicin-Loaded Apatite Cement/α-TCP Composites: An In Vitro Study.
Physical Properties and Antimicrobial Release Ability of Gentamicin-Loaded Apatite Cement/α-TCP Composites: An In Vitro Study.
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DOI：
10.3390/ma16030995
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发表时间：
2023-01-21
期刊：
影响因子：
3.4
通讯作者：
Aikawa, Tomonao
中科院分区：
文献类型：
--
作者：
Sasaki, Kazuki;Ninomiya, Yoshiaki;Takechi, Masaaki;Tsuru, Kanji;Ishikawa, Kunio;Shigeishi, Hideo;Ohta, Kouji;Aikawa, Tomonao
具有优良骨传导能力的磷灰石水泥（AC）和可用于骨替代的α-磷酸三钙（α-TCP）是有用的骨替代材料。本研究的目的是阐明负载抗生素的 AC/α-TCP 复合材料的物理性质和体外抗菌释放能力。以 2 种混合比（10:3 和 10:6）制备负载庆大霉素的快速凝固 AC/α-TCP 复合材料。在塑料模具中制备AC/α-TCP复合材料的水泥浆，并在37℃和100%相对湿度的恒温室中干燥24小时。进行径向拉伸强度测试、粉末X射线衍射分析和庆大霉素释放测试。 AC/α-TCP复合材料的径向拉伸强度明显低于单独的AC。粉末X射线衍射图显示AC/α-TCP复合材料和负载庆大霉素的AC/α-TCP复合材料中羟基磷灰石的特征峰。在负载庆大霉素的 AC/α-TCP 复合材料（10:3 和 10:6）中，释放的庆大霉素浓度一直保持在金黄色葡萄球菌的最低抑制浓度以上，直到第 30 天。我们的结果表明，负载庆大霉素的 AC/α-TCP 复合材料具有作为药物递送系统的潜力。进一步的研究对于研究负载庆大霉素的 AC/α-TCP 复合材料在动物模型中的抗菌活性和安全性至关重要。
Apatite cement (AC), which has excellent osteoconductive ability, and alpha-tricalcium phosphate (α-TCP), which can be used for bone replacement, are useful bone substitute materials. The objective of this study was to clarify the physical properties and antimicrobial release ability of antibiotic-loaded AC/α-TCP composites in vitro. Gentamicin-loaded, rapid setting AC/α-TCP composites were prepared in 2 mixing ratios (10:3 and 10:6). The cement paste of AC/α-TCP composites was prepared in a plastic mold and dried in a thermostatic chamber at 37 °C and 100% relative humidity for 24 h. A diametral tensile strength test, powder X-ray diffraction analysis, and gentamicin release test were performed. The diametral tensile strengths of the AC/α-TCP composites were significantly less than that of AC alone. Powder X-ray diffraction patterns exhibited the characteristic peaks of hydroxyapatite in the AC/α-TCP composites and gentamicin-loaded AC/α-TCP composites. The concentration of the released gentamicin was maintained above the minimum inhibitory concentration of Staphylococcus aureus until Day 30 in both the gentamicin-loaded AC/α-TCP composites (10:3 and 10:6). Our results suggest that a gentamicin-loaded AC/α-TCP composite has potential as a drug delivery system. Further study is essential to investigate the antimicrobial activity and safety of the gentamicin-loaded AC/α-TCP composites in animal models.
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影响因子：
5
作者：
Chen IC;Su CY;Nien WH;Huang TT;Huang CH;Lu YC;Chen YJ;Huang GC;Fang HW
通讯作者：
Fang HW
影响因子：
14
作者：
MIYAMOTO, Y;ISHIKAWA, K;ASAOKA, K
通讯作者：
ASAOKA, K
DOI：
10.3390/ma9040283
发表时间：
2016-04-13
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Takechi M;Ninomiya Y;Ohta K;Tada M;Sasaki K;Rahman MZ;Ohta A;Tsuru K;Ishikawa K
通讯作者：
Ishikawa K
DOI：
10.3390/ma11101993
发表时间：
2018-10-16
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Ishikawa K;Miyamoto Y;Tsuchiya A;Hayashi K;Tsuru K;Ohe G
通讯作者：
Ohe G
影响因子：
2.5
作者：
Takeyama, Hikaru;Maruta, Michito;Tsuru, Kanji
通讯作者：
Tsuru, Kanji