Celastrus aculeatus Merr. suppresses the induction and progression of autoimmune arthritis by modulating immune response to heat-shock protein 65.
Celastrus aculeatus Merr. suppresses the induction and progression of autoimmune arthritis by modulating immune response to heat-shock protein 65.
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DOI：
10.1186/ar2268
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发表时间：
2007
影响因子：
4.9
通讯作者：
Moudgil KD
中科院分区：
文献类型：
--
作者：
Tong L;Moudgil KD
补充和替代医学产品越来越多地用于治疗自身免疫性疾病。然而，这些药物的作用机制尚未完全确定。采用大鼠类风湿性关节炎（AA）模型，观察南蛇藤醇提物对AA大鼠关节炎的影响。南蛇藤（Celastrus）能降低AA的严重程度，并检测了南蛇藤诱导的对疾病相关抗原分枝杆菌热休克蛋白65（Bhsp65）的免疫应答的变化。通过用热灭活的M.结核病H37Ra（Mtb）。在Mtb攻击前（预防方案）或AA发作后（治疗方案）开始，每天通过管饲法向LEW大鼠饲喂南蛇藤。另一组大鼠给予甲氨蝶呤进行比较。定期对所有大鼠的关节炎体征进行分级。同时，对南蛇藤处理的大鼠的引流淋巴结细胞进行增殖和细胞因子反应测试，而对它们的血清进行炎性介质一氧化氮测试。南蛇藤喂养抑制AA的诱导以及进展，后者的效果与甲氨蝶呤相当。南蛇藤处理诱导细胞因子对抗炎类型的反应的相对偏差，并增强抗Bhsp65抗体的产生，已知抗Bhsp65抗体对AA具有保护作用。南蛇藤喂养也降低了一氧化氮的水平。根据我们的研究结果，我们建议进一步系统地探索南蛇藤作为类风湿关节炎的辅助治疗方式。
Complementary and alternative medicine products are increasingly being used for the treatment of autoimmune diseases. However, the mechanisms of action of these agents are not fully defined. Using the rat adjuvant arthritis (AA) model of human rheumatoid arthritis, we determined whether the ethanol extract of Celastrus aculeatus Merr. (Celastrus), a Chinese herb, can down-modulate the severity of AA, and also examined the Celastrus-induced changes in immune responses to the disease-related antigen mycobacterial heat-shock protein 65 (Bhsp65). AA was induced in the Lewis (LEW; RT.1l) rat by immunization subcutaneously with heat-killed M. tuberculosis H37Ra (Mtb). Celastrus was fed to LEW rats by gavage daily, beginning either before Mtb challenge (preventive regimen) or after the onset of AA (therapeutic regimen). An additional group of rats was given methotrexate for comparison. All rats were graded regularly for the signs of arthritis. In parallel, the draining lymph node cells of Celastrus-treated rats were tested for proliferative and cytokine responses, whereas their sera were tested for the inflammatory mediator nitric oxide. Celastrus feeding suppressed both the induction as well as the progression of AA, and the latter effect was comparable to that of methotrexate. Celastrus treatment induced relative deviation of the cytokine response to anti-inflammatory type and enhanced the production of anti-Bhsp65 antibodies, which are known to be protective against AA. Celastrus feeding also reduced the levels of nitric oxide. On the basis of our results, we suggest further systematic exploration of Celastrus as an adjunct therapeutic modality for rheumatoid arthritis.
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影响因子：
15.3
作者：
Moudgil, K D;Chang, T T;Eradat, H;Chen, A M;Gupta, R S;Brahn, E;Sercarz, E E
通讯作者：
Sercarz, E E
影响因子：
1.7
作者：
Guo, YQ;Li, X;Wang, JH
通讯作者：
Wang, JH
影响因子：
5.1
作者：
Kim, SE;Kim, YH;Kim, YC
通讯作者：
Kim, YC
DOI：
10.1128/cdli.12.5.575-580.2005
发表时间：
2005-05-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Chevrier, MR;Ryan, AE;Via, CS
通讯作者：
Via, CS
DOI：
10.1073/pnas.96.8.4524
发表时间：
1999-04-13
影响因子：
11.1
作者：
Haqqi, TM;Anthony, DD;Mukhtar, H
通讯作者：
Mukhtar, H