The secreted peptide PIP1 amplifies immunity through receptor-like kinase 7.
The secreted peptide PIP1 amplifies immunity through receptor-like kinase 7.
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分泌肽 PIP1 通过受体样激酶 7 增强免疫力
DOI：
10.1371/journal.ppat.1004331
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发表时间：
2014-09
期刊：
影响因子：
6.7
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Hou S;Wang X;Chen D;Yang X;Wang M;Turrà D;Di Pietro A;Zhang W
在植物中，位于质膜上的模式识别受体（PRR）在识别激发子后启动先天免疫应答，包括外源病原体相关分子模式（PAMP）和内源损伤相关分子模式（DAMP）。拟南芥产生超过1000种分泌肽候选物，但尚未确定这些候选物中是否有任何作为激发子。在这里，我们确定了一个A。拟南芥基因家族编码PAMP诱导的分泌肽（prePIP）的前体通过计算机方法。该家族的一些成员的表达，包括prePIP 1和prePIP 2，由多种病原体和诱导子诱导。亚细胞定位和蛋白水解加工分析表明，prePIP 1产物分泌到细胞外空间，在那里它被切割的C-末端。过表达prePIP 1和prePIP 2，或外源应用与prePIP 1和prePIP 2的C端保守区相对应的PIP 1和PIP 2合成肽，可增强A. thaliana.遗传和生化分析表明，受体样激酶7（RLK 7）作为PIP 1的受体发挥作用。一旦被RLK 7感知，PIP 1与DAMP PEP 1一起启动重叠和不同的免疫信号传导应答。PIP 1和PEP 1协同放大由PAMP鞭毛蛋白触发的免疫应答。总的来说，这些研究为拟南芥内源分泌肽的免疫调节提供了重要的见解。
In plants, innate immune responses are initiated by plasma membrane-located pattern recognition receptors (PRRs) upon recognition of elicitors, including exogenous pathogen-associated molecular patterns (PAMPs) and endogenous damage-associated molecular patterns (DAMPs). Arabidopsis thaliana produces more than 1000 secreted peptide candidates, but it has yet to be established whether any of these act as elicitors. Here we identified an A. thaliana gene family encoding precursors of PAMP-induced secreted peptides (prePIPs) through an in-silico approach. The expression of some members of the family, including prePIP1 and prePIP2, is induced by a variety of pathogens and elicitors. Subcellular localization and proteolytic processing analyses demonstrated that the prePIP1 product is secreted into extracellular spaces where it is cleaved at the C-terminus. Overexpression of prePIP1 and prePIP2, or exogenous application of PIP1 and PIP2 synthetic peptides corresponding to the C-terminal conserved regions in prePIP1 and prePIP2, enhanced immune responses and pathogen resistance in A. thaliana. Genetic and biochemical analyses suggested that the receptor-like kinase 7 (RLK7) functions as a receptor of PIP1. Once perceived by RLK7, PIP1 initiates overlapping and distinct immune signaling responses together with the DAMP PEP1. PIP1 and PEP1 cooperate in amplifying the immune responses triggered by the PAMP flagellin. Collectively, these studies provide significant insights into immune modulation by Arabidopsis endogenous secreted peptides.
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DOI：
10.1126/science.1244454
发表时间：
2014-01-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Haruta M;Sabat G;Stecker K;Minkoff BB;Sussman MR
通讯作者：
Sussman MR
影响因子：
64.8
作者：
Chinchilla, Delphine;Zipfel, Cyril;Boller, Thomas
通讯作者：
Boller, Thomas
影响因子：
56.9
作者：
Kondo, Tatsuhiko;Sawa, Shinichiro;Sakagami, Youji
通讯作者：
Sakagami, Youji
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1126/science.1171647
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Boller T;He SY
通讯作者：
He SY