Bacteriophage Protects Against Aerococcus viridans Infection in a Murine Mastitis Model
Bacteriophage Protects Against Aerococcus viridans Infection in a Murine Mastitis Model
复制标题
噬菌体可防止小鼠乳腺炎模型中的草绿色气球菌感染
DOI：
10.3389/fvets.2020.00588
复制
发表时间：
2020-08
影响因子：
3.2
通讯作者：
Jingmin Gu
中科院分区：
文献类型：
--
作者：
Hengyu Xi;Dali He;Dong Li;Shan-shan Liu;Gang Wang;Yalu Ji;Xinwu Wang;Zijing Wang;Lanting Bi;Rihong Zhao;Hao Zhang;Li Yang;Zhimin Guo;Wenyu Han;Jingmin Gu
奶牛乳房炎是一种常见于泌乳早期或干乳期的炎症性疾病，主要由细菌感染引起。越来越多的证据表明，Aerococcus viridans（A.草绿色）正在成为牛乳腺炎的重要原因。奶牛乳房炎的治疗主要是基于抗生素，这不仅导致了巨大的经济负担，而且还导致了抗生素耐药性的发展。另一方面，噬菌体提供了一种有前途的替代治疗策略。本研究的目的是评估以前分离的A.草绿色噬菌体vB_AviM_AVP（AVP）作为抗乳腺炎剂在实验A.草绿烷诱导的鼠乳腺炎模型。A.从临床牛乳腺炎的乳汁中分离出草绿色N14，并用于建立小鼠乳腺炎模型。我们证明，施用噬菌体AVP显著减少了A.绿色和减轻对乳房组织的损害。此外，与磷酸盐缓冲盐水（PBS）处理组相比，噬菌体处理组中炎性细胞因子（TNF-α、IL-1β和IL-6）和髓过氧化物酶（MPO）活性水平降低表明炎症减轻。据我们所知，这份报告是第一个显示潜在的使用，作为一个治疗A。草绿色菌素引起的乳腺炎。
Bovine mastitis, an inflammatory disease that occurs frequently in early lactation or the dry period, is primarily caused by bacterial infections. There is growing evidence that Aerococcus viridans (A. viridans) is becoming an important cause of bovine mastitis. The treatment of bovine mastitis is primarily based on antibiotics, which not only leads to a large economic burden but also the development of antibiotic resistance. On the other hand, bacteriophages present a promising alternative treatment strategy. The object of this study was to evaluate the potential of a previously isolated A. viridans phage vB_AviM_AVP (AVP) as an anti-mastitis agent in an experimental A. viridans-induced murine mastitis model. A. viridans N14 was isolated from the milk of clinical bovine mastitis and used to establish a mastitis model in mice. We demonstrated that administration of phage AVP significantly reduced colony formation by A. viridans and alleviated damage to breast tissue. In addition, reduced inflammation was indicated by decreased levels of inflammatory cytokines (TNF-α, IL-1β, and IL-6) and myeloperoxidase (MPO) activity in the phage-treated group compared to those in the phosphate buffered saline (PBS)-treated group. To the best of our knowledge, this report is the first to show the potential use of phages as a treatment for A. viridans-induced mastitis.
登录
查看更多内容
影响因子：
4.6
作者：
da Silva Duarte V;Dias RS;Kropinski AM;Campanaro S;Treu L;Siqueira C;Vieira MS;da Silva Paes I;Santana GR;Martins F;Crispim JS;da Silva Xavier A;Ferro CG;Vidigal PMP;da Silva CC;de Paula SO
通讯作者：
de Paula SO
影响因子：
4.2
作者：
Iwano H;Inoue Y;Takasago T;Kobayashi H;Furusawa T;Taniguchi K;Fujiki J;Yokota H;Usui M;Tanji Y;Hagiwara K;Higuchi H;Tamura Y
通讯作者：
Tamura Y
细菌感染治疗中的噬菌体疗法：发现噬菌体一百年后。
影响因子：
2.6
作者：
Cisek AA;Dąbrowska I;Gregorczyk KP;Wyżewski Z
通讯作者：
Wyżewski Z
DOI：
10.3390/molecules23040795
发表时间：
2018-03-30
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Manyi-Loh C;Mamphweli S;Meyer E;Okoh A
通讯作者：
Okoh A
利用 RNAseq 技术分析大肠杆菌引起的乳腺炎奶牛外周血 miRNA 的表达谱
影响因子：
4.6
作者：
Luoreng ZM;Wang XP;Mei CG;Zan LS
通讯作者：
Zan LS