Effects of the Probiotic, Lactobacillus delbrueckii subsp. bulgaricus, as a Substitute for Antibiotics on the Gastrointestinal Tract Microbiota and Metabolomics Profile of Female Growing-Finishing Pigs.
Effects of the Probiotic, Lactobacillus delbrueckii subsp. bulgaricus, as a Substitute for Antibiotics on the Gastrointestinal Tract Microbiota and Metabolomics Profile of Female Growing-Finishing Pigs.
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益生菌德氏乳杆菌亚种的作用。
DOI：
10.3390/ani12141778
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发表时间：
2022-07-11
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影响因子：
--
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中科院分区：
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德氏乳杆菌保加利亚亚种保加利亚乳杆菌（LDB）是猪生产中替代抗生素的重要候选菌株。本试验分别对生长肥育母猪饲喂低密度脂蛋白日粮和抗生素日粮。使用16 S rRNA测序来识别不同的微生物群。基于液相色谱-质谱的非靶向代谢组学方法用于鉴定不同的代谢物。分析了粪便微生物群和代谢产物的共现网络。结果包含猪生长性能、微生物群数据、代谢物数据和共现网络的信息，支持LDB作为猪生产中抗生素替代品的可能性。德氏乳杆菌保加利亚亚种保加利亚乳杆菌（LDB）是中国“批准的饲料添加剂”名单上批准的饲料添加剂。然而，LDB作为抗生素替代品的可能性仍不清楚。特别是，LDB对胃肠道（GIT）中微生物群和代谢物的影响需要进一步解释。本研究旨在鉴定粪便样品中存在的微生物群和代谢物，并研究微生物群和代谢物之间的关系，以评估LDB作为猪生产中抗生素替代品的潜力。将42头生长肥育母猪随机分为抗生素组（基础日粮+75mg/kg金霉素）和LDB组（基础日粮+3.0 × 109 cfu/kg LDB）。在第0天和第30天收集粪便样品。记录并评估生长性能。基于16 S rRNA测序和液相色谱-质谱的非靶向代谢组学方法用于分析微生物群和代谢物的差异。使用斯皮尔曼相关性和Benjamini-Hochberg调整计算差异之间的关联。LDB日粮对饲料转化率无不良影响，但对平均日增重和平均日采食量略有提高（p > 0.05）。日粮中添加LDB可提高乳酸菌的丰度，降低普雷沃氏菌属NK 3B 31类群的丰度。日粮中添加LDB提高了吡哆醇，酪胺，D-（+）-焦谷氨酸，次黄嘌呤，腐胺和5-羟基吲哚-3-乙酸的浓度，降低了石胆酸的浓度。在我们的研究中，乳酸菌网络（乳酸菌、消化球菌、瘤胃球菌科_UCG-004、土芝菌-志贺菌、苯乙酮、酪胺、腐胺、N-甲基异丙硫菌、N1-乙酰精胺）和普雷沃氏菌_NK3B31_群网络（普雷沃氏菌_NK3B31_群、密螺旋体_2、甘油单月桂酸酯、阿昔洛韦、N-（5-乙酰氨基戊基）乙酰胺、甘油3-磷酸）在LDB对猪GIT健康的影响中是最重要的。这些结果表明，LDB可能通过乳杆菌和Prevotellaceae_NK3B31_group网络调节GIT功能。但本研究结果仅限于生长肥育母猪的粪便样本，在评价LDB替代抗生素在猪生产中的应用时应综合考虑性别、生长阶段、品种等因素。
Lactobacillus delbrueckii subsp. bulgaricus (LDB) is an important candidate for antibiotic replacement in pig production. In this study, LDB and antibiotic diets were fed to the LDB and antibiotic groups of female growing-finishing pigs, respectively. 16S rRNA sequencing was used to identify different microbiota. Liquid chromatography-mass spectrometry-based non-targeted metabolomics approaches were used to identify different metabolites. The co-occurrence network of the fecal microbiota and metabolite was analyzed. The results contain information on pig growth performance, microbiota data, metabolite data and co-occurrence networks, supporting the possibility of LDB as an antibiotics replacement in pig production. Lactobacillus delbrueckii subsp. bulgaricus (LDB) is an approved feed additive on the Chinese ‘Approved Feed Additives’ list. However, the possibility of LDB as an antibiotic replacement remains unclear. Particularly, the effect of LDB on microbiota and metabolites in the gastrointestinal tract (GIT) requires further explanation. This study aimed to identify the microbiota and metabolites present in fecal samples and investigate the relationship between the microbiota and metabolites to evaluate the potential of LDB as an antibiotic replacement in pig production. A total of 42 female growing-finishing pigs were randomly allocated into the antibiotic group (basal diet + 75 mg/kg aureomycin) and LDB (basal diet + 3.0 × 109 cfu/kg LDB) groups. Fecal samples were collected on days 0 and 30. Growth performance was recorded and assessed. 16S rRNA sequencing and liquid chromatography-mass spectrometry-based non-targeted metabolomics approaches were used to analyze the differences in microbiota and metabolites. Associations between the differences were calculated using Spearman correlations with the Benjamini–Hochberg adjustment. The LDB diet had no adverse effect on feed efficiency but slightly enhanced the average daily weight gain and average daily feed intake (p > 0.05). The diet supplemented with LDB increased Lactobacillus abundance and decreased that of Prevotellaceae_NK3B31_group spp. Dietary-supplemented LDB enhanced the concentrations of pyridoxine, tyramine, D-(+)-pyroglutamic acid, hypoxanthine, putrescine and 5-hydroxyindole-3-acetic acid and decreased the lithocholic acid concentration. The Lactobacillus networks (Lactobacillus, Peptococcus, Ruminococcaceae_UCG-004, Escherichia-Shigella, acetophenone, tyramine, putrescine, N-methylisopelletierine, N1-acetylspermine) and Prevotellaceae_NK3B31_group networks (Prevotellaceae_NK3B31_group, Treponema_2, monolaurin, penciclovir, N-(5-acetamidopentyl)acetamide, glycerol 3-phosphate) were the most important in the LDB effect on pig GIT health in our study. These findings indicate that LDB may regulate GIT function through the Lactobacillus and Prevotellaceae_NK3B31_group networks. However, our results were restrained to fecal samples of female growing-finishing pigs; gender, growth stages, breeds and other factors should be considered to comprehensively assess LDB as an antibiotic replacement in pig production.
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影响因子：
4.6
作者：
Han GG;Lee JY;Jin GD;Park J;Choi YH;Kang SK;Chae BJ;Kim EB;Choi YJ
通讯作者：
Choi YJ
影响因子：
4
作者：
Jang, A-yeong;Rod-in, Weerawan;Park, Woo Jung
通讯作者：
Park, Woo Jung
影响因子：
4.5
作者：
通讯作者：
--
DOI：
10.1016/j.aninu.2016.11.002
发表时间：
2017-03
期刊：
Animal nutrition (Zhongguo xu mu shou yi xue hui)
影响因子：
--
作者：
Dowarah R;Verma AK;Agarwal N
通讯作者：
Agarwal N
影响因子：
5.2
作者：
Chen, Yung-Tsung;Chiou, Shiou-Yun;Lin, Jin-Seng
通讯作者：
Lin, Jin-Seng