Mucus organisation is shaped by colonic content; a new view.
Mucus organisation is shaped by colonic content; a new view.
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DOI：
10.1038/s41598-017-08938-3
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发表时间：
2017-08-17
影响因子：
4.6
通讯作者：
Theodorou V
中科院分区：
文献类型：
--
作者：
Kamphuis JBJ;Mercier-Bonin M;Eutamène H;Theodorou V
结肠粘液屏障通常被描述为覆盖在上皮细胞上的连续双层，将微生物群与肠道组织分开。该模型目前被认为在整个冒号中都有效。结肠的特点是局部解剖功能的特殊性，如内容物和位置的存在和一致性。在这项研究中，我们通过组织学和FISH染色来描述啮齿动物近端和远端结肠中结肠粘液屏障的组织结构，并考虑到上述的特殊性。通过使用具有和不具有结肠内容物的组织的纵向切片和成像，我们已经获得了结肠内粘液组织的时空概览。我们首次描述了结肠粘液层覆盖了粪便，而不是远端结肠的上皮。这种粪便粘液层将微生物群限制在粪便中，并防止其留在空远端结肠中。在近端结肠中，粘液没有在细菌和上皮之间形成隔离层。我们得出结论，结肠粘液的组织依赖于结肠内容物的存在和结肠内的位置。我们的发现重新开启了关于结肠粘液屏障性质的讨论。
The colonic mucus barrier is commonly described as a continuous double layer covering the epithelium, separating the microbiota from the intestinal tissue. This model is currently considered valid throughout the colon. The colon is characterised by regional anatomo-functional specificities such as presence and consistency of contents and location. In this study, we characterised the organisation of the colonic mucus barrier in proximal and distal colon of rodents by histological and FISH staining, taking into account aforementioned specificities. By using longitudinal sections and imaging extensive areas of tissue with and without colonic contents, we have obtained a spatiotemporal overview of mucus organisation in the colon. We describe for the first time that the colonic mucus layer covers the faeces instead of the epithelium in the distal colon. This faecal mucus layer confines the microbiota to the faeces and prevents it from remaining in empty distal colon. In the proximal colon, the mucus did not form a separating layer between bacteria and epithelium. We conclude that the organisation of colonic mucus is reliant on the presence of the colonic content, and the location within the colon. Our findings reopen the discussion on the nature of the colonic mucus barrier.
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影响因子：
30.3
作者：
Johansson ME;Jakobsson HE;Holmén-Larsson J;Schütte A;Ermund A;Rodríguez-Piñeiro AM;Arike L;Wising C;Svensson F;Bäckhed F;Hansson GC
通讯作者：
Hansson GC
影响因子：
6.7
作者：
Bergstrom KS;Kissoon-Singh V;Gibson DL;Ma C;Montero M;Sham HP;Ryz N;Huang T;Velcich A;Finlay BB;Chadee K;Vallance BA
通讯作者：
Vallance BA
DOI：
10.1152/ajpgi.2001.280.5.g922
发表时间：
2001-05-01
影响因子：
4.5
作者：
Atuma, C;Strugala, V;Holm, L
通讯作者：
Holm, L
DOI：
10.1016/s1095-6433(00)00183-5
发表时间：
2000-04-01
影响因子：
2.3
作者：
Shimotoyodome, A;Meguro, S;Sakata, T
通讯作者：
Sakata, T
影响因子：
7
作者：
Strugala, V;Allen, A;Pearson, JP
通讯作者：
Pearson, JP