Investigation of antiviral state mediated by interferon-inducible transmembrane protein 1 induced by H9N2 virus and inactivated viral particle in human endothelial cells.
Investigation of antiviral state mediated by interferon-inducible transmembrane protein 1 induced by H9N2 virus and inactivated viral particle in human endothelial cells.
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H9N2病毒和灭活病毒颗粒诱导人内皮细胞干扰素诱导跨膜蛋白1介导的抗病毒状态研究
DOI：
10.1186/s12985-017-0875-5
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发表时间：
2017-11-03
期刊：
影响因子：
4.8
通讯作者：
Qiao J
中科院分区：
文献类型：
--
作者：
Feng B;Zhao L;Wang W;Wang J;Wang H;Duan H;Zhang J;Qiao J
背景血管内皮细胞被认为在应对病毒感染中起着重要作用。我们先前的基因芯片分析表明，H9N2病毒感染和灭活病毒颗粒接种增加了人脐静脉内皮细胞(HUVECs)干扰素诱导的跨膜蛋白1(IFITM1)的表达。在本研究中，我们深入研究了H9N2病毒感染和灭活病毒颗粒接种诱导的人脐静脉内皮细胞中IFITM1和IFITM1介导的抗病毒反应的表达模式。方法首先，采用实时定量聚合酶链式反应和免疫印迹法检测H9N2病毒感染和病毒颗粒接种诱导的HUVECs中IFITM1的表达水平。其次，我们观察了血凝素或神经氨酸酶是否影响人脐静脉内皮细胞IFITM1的表达。结果H9N2病毒感染和病毒颗粒接种均可上调HUVECs中IFITM1的表达，但不增加干扰素ɑ/β的表达。HA或NA蛋白单独结合不足以增加HUVEC中IFITM1和干扰素-ɑ/β的水平。病毒颗粒接种诱导的IFITM1显著降低HUVEC培养上清液中的病毒滴度。结论灭活病毒颗粒接种后，IFITM1在mRNA和蛋白水平的表达均增加。此外，IFITM1表达的诱导介导了HUVECs的抗病毒状态。
BackgroundEndothelial cells are believed to play an important role in response to virus infection. Our previous microarray analysis showed that H9N2 virus infection and inactivated viral particle inoculation increased the expression of interferon-inducible transmembrane protein 1 (IFITM1) in human umbilical vein endothelial cells (HUVECs). In present study, we deeply investigated the expression patterns of IFITM1 and IFITM1-mediated antiviral response induced by H9N2 virus infection and inactivated viral particle inoculation in HUVECs. Epithelial cells that are considered target cells of the influenza virus were selected as a reference control.MethodsFirst, we quantified the expression levels of IFITM1 in HUVECs induced by H9N2 virus infection or viral particle inoculation using quantitative real-time PCR and western blot. Second, we observed whether hemagglutinin or neuraminidase affected IFITM1 expression in HUVECs. Finally, we investigated the effect of induced-IFITM1 on the antiviral state in HUVECs by siRNA and activation plasmid transfection.ResultsBoth H9N2 virus infection and viral particle inoculation increased the expression of IFITM1 without elevating the levels of interferon-ɑ/β in HUVECs. HA or NA protein binding alone is not sufficient to increase the levels of IFITM1 and interferon-ɑ/β in HUVECs. IFITM1 induced by viral particle inoculation significantly decreased the virus titers in culture supernatants of HUVECs.ConclusionsOur results showed that inactivated viral particle inoculation increased the expression of IFITM1 at mRNA and protein levels. Moreover, the induction of IFITM1 expression mediated the antiviral state in HUVECs.
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DOI：
10.1186/s40247-014-0011-6
发表时间：
2014-12
期刊：
Translational respiratory medicine
影响因子：
--
作者：
Herbert C;Zeng QX;Shanmugasundaram R;Garthwaite L;Oliver BG;Kumar RK
通讯作者：
Kumar RK
影响因子：
9.4
作者：
Butt, KM;Smith, GJD;Guan, Y
通讯作者：
Guan, Y
影响因子：
--
作者：
Dirix V;Verscheure V;Vermeulen F;De Schutter I;Goetghebuer T;Locht C;Mascart F
通讯作者：
Mascart F
DOI：
10.1073/pnas.160270697
发表时间：
2000-08-15
影响因子：
11.1
作者：
Lin, YP;Shaw, M;Hay, A
通讯作者：
Hay, A
影响因子：
1.6
作者：
Cong, Yan-Long;Wang, Chun-Feng;Liu, Jin-Hua
通讯作者：
Liu, Jin-Hua