Afamin Levels and Their Correlation with Oxidative and Lipid Parameters in Non-diabetic, Obese Patients.
Afamin Levels and Their Correlation with Oxidative and Lipid Parameters in Non-diabetic, Obese Patients.
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DOI：
10.3390/biom12010116
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发表时间：
2022-01-12
期刊：
影响因子：
5.5
通讯作者：
Paragh G
中科院分区：
文献类型：
--
作者：
Juhász I;Ujfalusi S;Seres I;Lőrincz H;Varga VE;Paragh G Jr;Somodi S;Harangi M;Paragh G
背景：Afamin 是一种肝脏产生的生物活性蛋白，具有 α-和 γ-生育酚结合位点。 Afamin 水平在代谢综合征和肥胖症中升高，并且与代谢综合征的组成部分密切相关。非糖尿病、肥胖患者中的 Afamin 浓度、Afamin 与维生素 E、Afamin 与脂蛋白亚组分之间的相关性尚未得到充分研究。方法：我们的研究涉及 50 名非糖尿病病态肥胖患者和 32 名健康、体重正常的个体。通过ELISA测量afamin浓度。通过凝胶电泳测定脂蛋白亚级分。气相色谱-质谱法用于测量α-和γ生育酚水平。结果：与健康对照相比，肥胖患者的 Afamin 浓度显着较高（70.4 ± 12.8 vs. 47.6 ± 8.5 μg/mL，p < 0.001）。研究发现，afamin 与空腹血糖、HbA1c、hsCRP、甘油三酯、氧化 LDL 水平以及小 HDL 亚组分的数量和比例呈正相关。在 afamin 和平均 LDL 大小以及大 HDL 亚组分的数量和比例之间观察到负相关。经过多元回归分析，HbA1c 水平和小 HDL 被证明是 afamin 的独立预测因子。结论：Afamin 可能通过胰岛素抵抗的发展而不是通过影响 α-和 γ-生育酚水平参与肥胖相关氧化应激的发展。
Background: Afamin is a liver-produced bioactive protein and features α- and γ-tocopherol binding sites. Afamin levels are elevated in metabolic syndrome and obesity and correlate well with components of metabolic syndrome. Afamin concentrations, correlations between afamin and vitamin E, afamin and lipoprotein subfractions in non-diabetic, obese patients have not been fully examined. Methods: Fifty non-diabetic, morbidly obese patients and thirty-two healthy, normal-weight individuals were involved in our study. The afamin concentrations were measured by ELISA. Lipoprotein subfractions were determined with gel electrophoresis. Gas chromatography–mass spectrometry was used to measure α- and γ tocopherol levels. Results: Afamin concentrations were significantly higher in the obese patients compared to the healthy control (70.4 ± 12.8 vs. 47.6 ± 8.5 μg/mL, p < 0.001). Positive correlations were found between afamin and fasting glucose, HbA1c, hsCRP, triglyceride, and oxidized LDL level, as well as the amount and ratio of small HDL subfractions. Negative correlations were observed between afamin and mean LDL size, as well as the amount and ratio of large HDL subfractions. After multiple regression analysis, HbA1c levels and small HDL turned out to be independent predictors of afamin. Conclusions: Afamin may be involved in the development of obesity-related oxidative stress via the development of insulin resistance and not by affecting α- and γ-tocopherol levels.
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影响因子：
9.8
作者：
BARAKAT, HA;MCLENDON, VD;CARPENTER, JW
通讯作者：
CARPENTER, JW
影响因子：
5.5
作者：
Camps J;Castañé H;Rodríguez-Tomàs E;Baiges-Gaya G;Hernández-Aguilera A;Arenas M;Iftimie S;Joven J
通讯作者：
Joven J
影响因子：
37.8
作者：
Hodis, HN;Mack, WJ;Azen, SP
通讯作者：
Azen, SP
影响因子：
5.9
作者：
Klop B;Elte JW;Cabezas MC
通讯作者：
Cabezas MC
DOI：
10.1111/j.1749-6632.1972.tb27853.x
发表时间：
1972-01-01
影响因子：
5.2
作者：
GREEN, J
通讯作者：
GREEN, J