Locally collateralizing glutamate neurons in the dorsal raphe nucleus responsive to substance P contain vesicular glutamate transporter 3 (VGLUT3).
Locally collateralizing glutamate neurons in the dorsal raphe nucleus responsive to substance P contain vesicular glutamate transporter 3 (VGLUT3).
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反应于物质P的背raphe核中局部副谷氨酸神经元包含囊泡谷氨酸转运蛋白3（VGLUT3）。
DOI：
10.1016/j.jchemneu.2009.05.005
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发表时间：
2009-12
影响因子：
2.8
通讯作者：
Commons KG
中科院分区：
文献类型：
--
作者：
Commons KG
5 -羟色胺能中缝背核（DR）是一个富含细胞体和轴突的区域，其中含有第三个泡状谷氨酸转运体VGLUT3。然而，含有vglut3的轴突在调节DR内5-羟色胺（5-HT）神经元活性中的作用仍然知之甚少。本研究检测了DR中含vglut3的细胞体和轴突的神经化学特征和形貌。由于许多5-HT细胞已被报道表达VGLUT3，双标记轴突在DR内的分布被检查，含有VGLUT3和5-HT免疫标记的轴突具有地形分布：它们支配室管膜，并在导水管底部的尾侧DR内分支，该区域已知引起室管膜神经支配。因此，VGLUT3仅存在于复发性5-HT轴突侧枝的特定子组分中。剩余的VGLUT3轴突很少被单胺、GABA或乙酰胆碱标记物双重免疫标记，这表明这些轴突主要含有谷氨酸填充的囊泡。由于P物质受体神经激肽1 （NK1）先前与DR中的局部谷氨酸神经元相关，因此使用三重免疫标记检测了NK1, 5-HT和VGLUT3细胞之间的关系。结果表明，DR中大多数非5- ht含vglut3的细胞体通常含有NK1免疫标记。综上所述，这些发现表明对P物质有反应的局部侧支谷氨酸神经元含有VGLUT3。
The serotonergic dorsal raphe nucleus (DR) is an area enriched with cell bodies and axons containing the third vesicular glutamate transporter, VGLUT3. However, the role of VGLUT3-containing axons in modulating activity of serotonin (5-HT) neurons within the DR remains poorly understood. In this study, neurochemical features and topography of VGLUT3-containing cell bodies and axons in the DR were examined. Since many 5-HT cells have been reported to express VGLUT3, the distribution of dually labeled axons was examined within the DR. Axons containing both VGLUT3 and 5-HT immunolabeling had a topographic distribution: they innervated the ependyma and ramified within the caudal DR at the base of the aqueduct, an area known to give rise to ependymal innervation. Thus VGLUT3 is only present in a specific subcomponent of recurrent 5-HT axon collaterals. Remaining VGLUT3 axons were only rarely dually-immunolabeled for markers of monoamines, GABA, or acetycholine, suggesting these axons contain a predominance of glutamate-filled vesicles. Since the substance P receptor, neurokinin 1 (NK1), has previously been associated with local glutamate neurons in the DR, the relationship between NK1, 5-HT and VGLUT3 cells was examined using triple-immunolabeling. Results indicate that the majority of non-5-HT VGLUT3-containing cell bodies in the DR often contain NK1 immunolabeling. Taken together, these findings indicate locally collateralizing glutamate neurons responsive to substance P contain VGLUT3.
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影响因子：
2.5
作者：
Boulland, JL;Qureshi, T;Chaudhry, FA
通讯作者：
Chaudhry, FA
影响因子：
3.4
作者：
Gabellec, Marie-Madeleine;Panzanelli, Patrizia;Lledo, Pierre-Marie
通讯作者：
Lledo, Pierre-Marie
影响因子：
2.5
作者：
Mintz, EM;Scott, TJ
通讯作者：
Scott, TJ
影响因子：
--
作者：
Michelsen, Kimmo A.;Schmitz, Christoph;Steinbusch, Harry W. M.
通讯作者：
Steinbusch, Harry W. M.
影响因子：
16.2
作者：
Fremeau, RT;Troyer, MD;Edwards, RH
通讯作者：
Edwards, RH