Dysbiosis of small intestinal microbiota in liver cirrhosis and its association with etiology.
Dysbiosis of small intestinal microbiota in liver cirrhosis and its association with etiology.
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肝硬化小肠菌群失调及其与病因的关系
DOI：
10.1038/srep34055
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发表时间：
2016-09-30
影响因子：
4.6
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Chen Y;Ji F;Guo J;Shi D;Fang D;Li L
肝硬化相关的十二指肠生态失调尚未明确定义。本研究采用16S rRNA基因焦磷酸测序方法分析了30例肝硬化患者和28例健康对照者的十二指肠黏膜微生物群。主坐标分析显示，与对照组相比，肝硬化患者的十二指肠黏膜微生物群存在显著差异。在属水平上，在肝硬化十二指肠中发现了过多的Veillonella，Megasphaera,Dialister，Atopobium和prevotella。健康对照组的十二指肠微生物群富含奈瑟菌、嗜血杆菌和sr1菌属。另一方面，根据预测的宏基因组分析，肝硬化十二指肠微生物群中与营养吸收（如糖和氨基酸代谢）相关的基因通路高度丰富，而与细菌增殖和定植相关的功能模块（如细菌运动蛋白和分泌系统）在对照组中被过度代表。当考虑肝硬化的病因时，发现两个操作分类单位（otu）， OTU-23（奈瑟菌）和OTU-36 (Gemella)，可以区分乙型肝炎病毒相关肝硬化和原发性胆汁性肝硬化。结果提示肝硬化患者的十二指肠黏膜微生物群结构与健康对照组有显著差异。十二指肠生态失调可能与口腔菌群的改变和十二指肠微环境的改变有关。
Cirrhosis-associated duodenal dysbiosis is not yet clearly defined. In this research, duodenal mucosal microbiota was analyzed in 30 cirrhotic patients and 28 healthy controls using 16S rRNA gene pyrosequencing methods. The principal coordinate analysis revealed that cirrhotic patients were colonized by remarkable different duodenal mucosal microbiota in comparison with controls. At the genus level,Veillonella,Megasphaera,Dialister,Atopobium, andPrevotellawere found overrepresented in cirrhotic duodenum. And the duodenal microbiota of healthy controls was enriched withNeisseria,Haemophilus, andSR1 genera incertae sedis. On the other hand, based on predicted metagenomes analyzed, gene pathways related to nutrient absorption (e.g. sugar and amino acid metabolism) were highly abundant in cirrhosis duodenal microbiota, and functional modules involved in bacterial proliferation and colonization (e.g. bacterial motility proteins and secretion system) were overrepresented in controls. When considering the etiology of cirrhosis, two operational taxonomic units (OTUs), OTU-23 (Neisseria) and OTU-36 (Gemella), were found discriminative between hepatitis-B-virus related cirrhosis and primary biliary cirrhosis. The results suggest that the structure of duodenal mucosa microbiota in cirrhotic patients is dramatically different from healthy controls. The duodenum dysbiosis might be related to alterations of oral microbiota and changes in duodenal micro-environment.
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影响因子：
3.7
作者：
Chen W;Liu F;Ling Z;Tong X;Xiang C
通讯作者：
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影响因子：
13.5
作者：
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13.5
作者：
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作者：
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Stoneking, Mark
DOI：
10.1099/00207713-50-3-979
发表时间：
2000-05-01
影响因子：
2.8
作者：
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通讯作者：
Wade, WG