Transcription through enhancers suppresses their activity in Drosophila.
Transcription through enhancers suppresses their activity in Drosophila.
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DOI：
10.1186/1756-8935-6-31
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发表时间：
2013-09-26
影响因子：
3.9
通讯作者：
Chetverina D
中科院分区：
文献类型：
--
作者：
Erokhin M;Davydova A;Parshikov A;Studitsky VM;Georgiev P;Chetverina D
增强子元件以组织特异性方式决定靶基因转录的水平，从而提供不同细胞中基因表达的个体模式。控制增强子作用的机制的知识对于理解转录的全局调节是至关重要的。特别地，增强子通常位于基因组的转录区域内。许多实验表明，转录对调控元件既有积极的影响，也有消极的影响。在这项研究中，我们进行了直接测试的转录增强子活性的影响。使用转基因报告系统，我们研究了通过转录的存在和果蝇增强子控制的白色和黄色基因的表达的活性之间的关系。结果表明，来自不同启动子的转录影响增强子的活性，抵消了它们激活靶基因的能力。正如预期的那样，在抑制性启动子和受影响的增强子之间存在转录终止子强烈地降低了抑制。此外，转录导致Zeste蛋白的移位，Zeste蛋白负责白色基因的增强子依赖性调节，这表明这种调节的“转录干扰”机制。我们的研究结果表明，通过转录的增强子活性的负调控的作用。
Enhancer elements determine the level of target gene transcription in a tissue-specific manner, providing for individual patterns of gene expression in different cells. Knowledge of the mechanisms controlling enhancer action is crucial for understanding global regulation of transcription. In particular, enhancers are often localized within transcribed regions of the genome. A number of experiments suggest that transcription can have both positive and negative effects on regulatory elements. In this study, we performed direct tests for the effect of transcription on enhancer activity. Using a transgenic reporter system, we investigated the relationship between the presence of pass-through transcription and the activity of Drosophila enhancers controlling the expression of the white and yellow genes. The results show that transcription from different promoters affects the activity of enhancers, counteracting their ability to activate the target genes. As expected, the presence of a transcriptional terminator between the inhibiting promoter and the affected enhancer strongly reduces the suppression. Moreover, transcription leads to dislodging of the Zeste protein that is responsible for the enhancer-dependent regulation of the white gene, suggesting a 'transcription interference’ mechanism for this regulation. Our findings suggest a role for pass-through transcription in negative regulation of enhancer activity.
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影响因子：
56.9
作者：
Cheng, J;Kapranov, P;Gingeras, TR
通讯作者：
Gingeras, TR
影响因子：
16
作者：
Kanhere A;Viiri K;Araújo CC;Rasaiyaah J;Bouwman RD;Whyte WA;Pereira CF;Brookes E;Walker K;Bell GW;Pombo A;Fisher AG;Young RA;Jenner RG
通讯作者：
Jenner RG
影响因子：
56.9
作者：
Kapranov, Philipp;Cheng, Jill;Gingeras, Thomas R.
通讯作者：
Gingeras, Thomas R.
影响因子：
56.9
作者：
Bertone, P;Stolc, V;Snyder, M
通讯作者：
Snyder, M
影响因子：
10.5
作者：
GEYER, PK;CORCES, VG
通讯作者：
CORCES, VG