Characterization of the development of Haemonchus contortus ZJ strain from gerbils.
Characterization of the development of Haemonchus contortus ZJ strain from gerbils.
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沙鼠捻转血矛线虫 ZJ 菌株的发育特征
DOI：
10.1186/s13071-017-2465-1
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发表时间：
2017-10-23
影响因子：
3.2
通讯作者：
Du A
中科院分区：
文献类型：
--
作者：
Yang Y;Guo X;Zhang H;Huang Y;Chen X;Du A
缠绕血圆线虫是一种严重寄生于世界各地家畜体内的线虫，中国也不例外。缠绕血圆线虫对商业驱虫剂产生了广泛的抗药性，这就产生了对新的防治方法的需求，如更有效的药物。感染了螺旋藻的沙鼠以前曾被用作驱虫剂选择的模型，并对螺旋藻的生长进行了简要的研究。为了加强模型，这项研究提供了一个额外的描述螺旋杆菌ZJ株在沙土鼠体内的发展。以2000只脱鞘感染幼虫(XL3s)感染沙土鼠，分别于感染后4、7、18d处死沙土鼠。胃内仅发现四期幼虫。在三个采样时间点中的每个时间点都获得了约2%的接种量。幼虫在沙土鼠体内的生长速度慢于在绵羊体内，但呈现出几乎相同的形态。幼虫肠道细胞内有棒状晶状包裹体，表明幼虫的代谢率可能大大降低。胃切片组织学检查显示，幼虫位于管腔或粘膜表面，炎性改变不明显。本文介绍了螺旋体血吸虫ZJ株在沙土鼠体内的发育及特性。我们的结果为螺旋藻在沙土鼠体内的生长，特别是棒状晶体包裹体的存在提供了补充信息，并可能有助于改进驱虫药的选择体系。
Haemonchus contortus is a serious parasitic nematode in domestic ruminants around the world, including China. Haemonchus contortus has developed extensive resistance to commercial anthelmintics, which has produced a demand for new control methods, such as more effective drugs. Gerbils infected with H. contortus have previously been used as a model for anthelmintics selections, and the growth of H. contortus had been briefly examined. To enhance the model, this study provides an additional description of the development of H. contortus ZJ strain in gerbils. Gerbils were infected with H. contortus ZJ strain at a dose of 2000 exsheathed infective larvae (xL3s) and sacrificed at 4, 7 and 18 days post-infection (dpi). Only fourth-stage larvae were found in the stomachs. About 2% of the inoculums were obtained at each of the three sampling time points. Larvae grew more slowly in gerbils than in sheep, but presented almost the same morphology. Rod-like crystalline inclusions were present in the intestinal cells of larvae, indicating that the metabolic rate of larvae was probably greatly reduced. Histological examination of stomach sections showed that larvae are located in the lumens or at the mucosal surfaces, with few inflammatory changes evident. The development and features of H. contortus ZJ strain in gerbils were described. Our results provide supplementary information of H. contortus growth in gerbils, especially the presence of rod-like crystalline inclusions, and may contribute to improve the anthelmintic selection system.
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影响因子：
1.3
作者：
SOMMERVILLE, RI
通讯作者：
SOMMERVILLE, RI
影响因子：
2.6
作者：
Squires, Jill M.;Foster, Joyce G.;Zajac, Anne M.
通讯作者：
Zajac, Anne M.
影响因子：
4
作者：
ROTHWELL, JT;SANGSTER, NC
通讯作者：
SANGSTER, NC
圆线虫新基因 Hc-daf-22 的结构和功能特征
影响因子：
3.2
作者：
Guo X;Zhang H;Zheng X;Zhou Q;Yang Y;Chen X;Du A
通讯作者：
Du A
影响因子：
2.4
作者：
MARTIN, J;LEE, DL
通讯作者：
LEE, DL