Gene delivery into plant cells for recombinant protein production.
Gene delivery into plant cells for recombinant protein production.
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DOI：
10.1155/2015/932161
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发表时间：
2015
影响因子：
--
通讯作者：
Lai H
中科院分区：
文献类型：
--
作者：
Chen Q;Lai H
重组蛋白主要来自哺乳动物、昆虫和细菌细胞的培养。近年来，解构型病毒载体的发展使植物成为重组蛋白生产的可行平台，与现有的生产模式相比，植物在通用性、速度、成本、可扩展性和安全性方面具有优势。本文综述了农业渗透方法的最新进展，这是一种克服转基因进入植物细胞大规模生产重组蛋白的挑战的解决方案。首先总结了植物基因传递的一般方法，然后对每种农业入渗方法的应用和可扩展性进行了广泛的讨论。重点介绍了喷雾型土壤入渗技术的新进展及其在大田植物上的应用。农业渗透载体的讨论主要集中在它们在生产复杂和异多聚体蛋白质方面的应用，并随着桥载体的发展而更新。随后展示了在烟草属和非烟草属植物宿主中农业渗透的进展，以及它们在生产高价值人类生物制品和低成本、大批量工业酶方面的应用。这些在农业渗透方面的新进展极大地提高了转基因在植物中传递的稳健性和可扩展性，促进了植物瞬时表达系统的采用，以制造具有广泛应用的重组蛋白。
Recombinant proteins are primarily produced from cultures of mammalian, insect, and bacteria cells. In recent years, the development of deconstructed virus-based vectors has allowed plants to become a viable platform for recombinant protein production, with advantages in versatility, speed, cost, scalability, and safety over the current production paradigms. In this paper, we review the recent progress in the methodology of agroinfiltration, a solution to overcome the challenge of transgene delivery into plant cells for large-scale manufacturing of recombinant proteins. General gene delivery methodologies in plants are first summarized, followed by extensive discussion on the application and scalability of each agroinfiltration method. New development of a spray-based agroinfiltration and its application on field-grown plants is highlighted. The discussion of agroinfiltration vectors focuses on their applications for producing complex and heteromultimeric proteins and is updated with the development of bridge vectors. Progress on agroinfiltration in Nicotiana and non-Nicotiana plant hosts is subsequently showcased in context of their applications for producing high-value human biologics and low-cost and high-volume industrial enzymes. These new advancements in agroinfiltration greatly enhance the robustness and scalability of transgene delivery in plants, facilitating the adoption of plant transient expression systems for manufacturing recombinant proteins with a broad range of applications.
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影响因子：
4
作者：
Ca単izares, MC;Nicholson, L;Lomonossoff, GP
通讯作者：
Lomonossoff, GP
影响因子：
50.5
作者：
Bendandi, M.;Marillonnet, S.;Gleba, Y.
通讯作者：
Gleba, Y.
DOI：
10.1073/pnas.83.10.3282
发表时间：
1986-05-01
影响因子：
11.1
作者：
GRIMSLEY, N;HOHN, B;WALDEN, R
通讯作者：
WALDEN, R
DOI：
10.1073/pnas.0606631103
发表时间：
2006-10-03
影响因子：
11.1
作者：
Giritch, Anatoli;Marillonnet, Sylvestre;Gleba, Yuri
通讯作者：
Gleba, Yuri
DOI：
10.1079/9781780643434.0042
发表时间：
2014-01-01
期刊：
PLANT-DERIVED PHARMACEUTICALS: PRINCIPLES AND APPLICATIONS FOR DEVELOPING COUNTRIES
影响因子：
--
作者：
Chen, Qiang;Lai, Huafang
通讯作者：
Lai, Huafang