Nanoresonator chip-based RNA sensor strategy for detection of circulating tumor cells: response using PCA3 as a prostate cancer marker.
Nanoresonator chip-based RNA sensor strategy for detection of circulating tumor cells: response using PCA3 as a prostate cancer marker.
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DOI：
10.1016/j.nano.2011.11.009
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发表时间：
2012-08
影响因子：
5.4
通讯作者：
Clawson, Gary A.
中科院分区：
文献类型：
--
作者：
Sioss, James A.;Bhiladvala, Rustom B.;Pan, Weihua;Li, Mingwei;Patrick, Susan;Xin, Ping;Dean, Stacey L.;Keating, Christine D.;Mayer, Theresa S.;Clawson, Gary A.
血液中的循环肿瘤细胞（CTC）受到广泛关注。CTC的直接检测（通常< 1/ml）由于许多因素而复杂化，但是每个CTC存在约103至104个拷贝的靶RNA，加上简单的富集，可以大大提高检测能力。在这里，我们使用的共振频率偏移引起的质量放大金纳米粒子，检测结合到功能化的纳米线的杂交三明治。我们选择PCA 3 RNA作为前列腺癌的标志物，优化反义结合位点并定义允许单核苷酸错配歧视的条件，并使用混合谐振器集成方案，该方案将自上而下制造的元素与自下而上制造的优势相结合，以实现多路复用传感。根据频率偏移计算的结合质量与通过计数金纳米颗粒估计的质量相匹配。这代表了使用这种纳米谐振器的第一次演示，这种纳米谐振器显示出优异的特异性和定量灵敏度。
There is widespread interest in circulating tumor cells (CTCs) in blood. Direct detection of CTCs (often < 1/ml) is complicated by a number of factors, but the presence of ~103 to 104 copies of target RNA per CTC, coupled with simple enrichments, can greatly increase detection capability. Here we used resonance frequency shifts induced by mass-amplifying gold nanoparticles, to detect a hybridization sandwich bound to functionalized nanowires. We selected PCA3 RNA as a marker for prostate cancer, optimized antisense binding sites and defined conditions allowing single nucleotide mismatch discrimination, and used a hybrid resonator integration scheme which combines elements of top-down fabrication with strengths of bottom-up fabrication, with a view to enable multiplexed sensing. Bound mass calculated from frequency shifts matched mass estimated by counting gold nanoparticles. This represents the first demonstration of use of such nanoresonators, which show promise of both excellent specificity and quantitative sensitivity.
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