Surface biofunctionalization by covalent co-immobilization of oligopeptides.
Surface biofunctionalization by covalent co-immobilization of oligopeptides.
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DOI：
10.1016/j.colsurfb.2013.02.005
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发表时间：
2013-07-01
期刊：
影响因子：
--
通讯作者：
Aparicio C
中科院分区：
文献类型：
--
作者：
Chen X;Sevilla P;Aparicio C
具有多种生物活性的植入物功能化是获得具有改善的生物和临床性能的表面所需要的。我们的目标是开发一种简单可靠的方法来获得含有不同寡肽的稳定多功能涂层。我们用一种简单可靠的方法在钛表面共固定已知的具有协同生物活性的寡肽。将适当设计的含有RGD或PHSRN生物活性序列的寡肽混合并在cptes -硅化表面上共价结合。制备了仅由两种所研究肽中的一种制成的涂层和物理吸附寡肽的涂层作为对照组进行了测试。我们在涂层制备的每个步骤之后以及在机械挑战获得的涂层之后对获得的表面进行了彻底的表征。用不同颜色的荧光探针对RGD和PHSRN肽进行荧光标记，可以直接看到寡肽的共固定过程。我们证明了涂层是机械稳定的。与对照表面相比，共固定RGD和PHSRN肽的表面显著改善了成骨细胞的反应，这评估了我们的涂层方法对种植体表面生物激活的有效性。同样简单的方法可以通过共固定具有不同靶向生物活性（细胞募集和分化、生物矿物成核、抗菌活性）的寡肽来获得其他多功能表面，从而进一步提高钛种植体的临床性能。
Functionalization of implants with multiple bioactivities is desired to obtain surfaces with improved biological and clinical performance. Our objective was developing a simple and reliable method to obtain stable multifunctional coatings incorporating different oligopeptides. We co-immobilized on titanium surface oligopeptides of known cooperative bioactivities with a simple and reliable method. Appropriately designed oligopeptides containing either RGD or PHSRN bioactive sequences were mixed and covalently bonded on CPTES-silanized surfaces. Coatings made of only one of the two investigated peptides and coatings with physisorbed oligopeptides were produced and tested as control groups. We performed thorough characterization of the obtained surfaces after each step of the coating preparation and after mechanically challenging the obtained coatings. Fluorescence labeling of RGD and PHSRN peptides with fluorescence probes of different colors enabled the direct visualization of the co-immobilization of the oligopeptides. We proved that the coatings were mechanically stable. The surfaces with co-immobilized RGD and PHSRN peptides significantly improved osteoblasts response in comparison with control surfaces, which assessed the effectiveness of our coating method to bio-activate the implant surfaces. This same simple method can be used to obtain other multi-functional surfaces by co-immobilizing oligopeptides with different targeted bioactivities – cell recruitment and differentiation, biomineral nucleation, antimicrobial activity – and thus, further improving the clinical performance of titanium implants.
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影响因子：
3.2
作者：
Auernheimer, J;Zukowski, D;Kessler, H
通讯作者：
Kessler, H
DOI：
10.1002/jbm.b.30579
发表时间：
2007-01-01
影响因子：
3.4
作者：
Michael, J.;Beutner, R.;Schwenzer, B.
通讯作者：
Schwenzer, B.
影响因子：
14
作者：
Kim, Sung Eun;Song, Sang-Hun;Kwon, Yong-Dae
通讯作者：
Kwon, Yong-Dae
DOI：
10.1073/pnas.78.6.3824
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HOPP, TP;WOODS, KR
通讯作者：
WOODS, KR
影响因子：
4.1
作者：
Lucke, M;Schmidmaier, G;Raschke, M
通讯作者：
Raschke, M