Effective tuning of ligand incorporation and mechanical properties in visible light photopolymerized poly(ethylene glycol) diacrylate hydrogels dictates cell adhesion and proliferation.
Effective tuning of ligand incorporation and mechanical properties in visible light photopolymerized poly(ethylene glycol) diacrylate hydrogels dictates cell adhesion and proliferation.
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在可见光光聚聚合聚（乙二醇）二烯酸酯水凝胶中，配体掺入和机械性能的有效调整决定细胞粘附和增殖。
DOI：
10.1088/1748-6041/8/2/025001
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发表时间：
2013-04
期刊：
影响因子：
--
通讯作者：
Papavasiliou G
中科院分区：
文献类型：
--
作者：
Turturro MV;Sokic S;Larson JC;Papavasiliou G
细胞行为是由基质机械性质以及可溶性和固定化生化信号的复杂相互作用指导的。合成支架的发展，包括天然细胞外基质（ECM）的支持细胞增殖和组织再生的关键功能，需要这些生物材料内的ECM信号的刚度和固定浓度进行调整和优化之前，在体外和体内研究。进行了详细的实验灵敏度分析，以确定在可见光光聚合的聚（乙二醇）二丙烯酸酯（PEGDA）水凝胶内的弹性模量和固定化YRGDS显着变化的关键聚合条件。在所研究的聚合条件中，N-乙烯基吡咯烷酮（NVP）和丙烯基-PEG 3400-YRGDS的前体浓度的单一变化以及同时变化导致宽范围的水凝胶弹性模量（81 - 1178 kPa）和YRGDS表面浓度（0.04 - 1.72 pmol/cm 2）。增加YRGDS表面浓度增强成纤维细胞的粘附和增殖，对于给定的刚度，而水凝胶弹性模量的增加引起细胞粘附的减少和增殖的增加。关键聚合条件的确定对于生物材料性质的调整和优化以及细胞-基质相互作用的受控研究至关重要。
Cell behavior is guided by the complex interplay of matrix mechanical properties as well as soluble and immobilized biochemical signals. The development of synthetic scaffolds that incorporate key functionalities of the native extracellular matrix (ECM) for support of cell proliferation and tissue regeneration requires that stiffness and immobilized concentrations of ECM signals within these biomaterials be tuned and optimized prior to in vitro and in vivo studies. A detailed experimental sensitivity analysis was conducted to identify the key polymerization conditions that result in significant changes in both elastic modulus and immobilized YRGDS within visible light photopolymerized poly(ethylene glycol) diacrylate (PEGDA) hydrogels. Among the polymerization conditions investigated, single as well as simultaneous variations in N-vinylpyrrolidinone (NVP) and precursor concentrations of Acryl-PEG3400-YRGDS resulted in a broad range of hydrogel elastic modulus (81 – 1178 kPa) and YRGDS surface concentration (0.04 – 1.72 pmol/cm2). Increasing the YRGDS surface concentration enhanced fibroblast cell adhesion and proliferation for a given stiffness, while increases in hydrogel elastic modulus caused decreases in cell adhesion and increases in proliferation. The identification of key polymerization conditions is critical for the tuning and optimization of biomaterial properties and the controlled study of cell-substrate interactions.
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